2015-2016年浙江省台州市感染性腹泻轮状病毒分子流行病学研究

Molecular epidemiology of rotavirus in infectious diarrhea patients in Taizhou,2015-2016

目的分析2015—2016年浙江省台州市腹泻病例轮状病毒感染的流行病学特征、病毒基因型别及变化特征,为感染性腹泻的预防与控制提供实验室支持。方法收集2015—2016年台州市哨点医院感染性腹泻就诊患者信息及粪便标本,采用双重荧光方法检测A/B组轮状病毒,对A组轮状病毒阳性的标本进行G/P基因分型和测序,并对主要型别的VP7基因进行同源性和系统进化分析。结果轮状病毒总检出率为5.10%(81/1 589),其中A组轮状病毒占98.77%(80/81),B组轮状病毒占1.23%(1/81)。流行特征显示年龄、季节、职业等差异有统计学意义,性别和籍贯等差异无统计学意义。A组轮状病毒基因型别主要包括G9P[8](53.75%)、G2P[4](25.00%)、G3P[8](11.25%)、G1P[8](2.50%)、G4P[6](2.50%)。VP7基因同源性和进化分析显示,G9P[8]型主要是G9-Ⅵ亚型,相同基因型轮状病毒同源性高。结论轮状病毒是台州市感染性腹泻人群中的常见病原体; A组轮状病毒在本地区呈现多样性和稳定性。建议将G9型引入疫苗株,并对腹泻人群尤其是儿童进行持续性的病原学监测。

Objective To analyze the epidemiological characteristics of rotavirus infection and molecular characteristics of rotavirus in Taizhou, Zhejiang province. Methods Stool samples and information of patients with infectious diarrhea were collected from 2 sentinel hospitals in Taizhou from 2015–2016. The dual-fluorescence PCR was used to detect A/B group rotaviruses. The G/P genotyping and sequencing of all group A rotavirus-positive samples were performed. The homology and phylogenetic relationship of VP7 gene of major types were analyzed. Results Eighty-one strains of rotaviruses were detected in 1 589 diarrhea samples（5.10%）, in which group A rotavirus accounted for 98.77%（80/81） and group B rotavirus accounted for 1.23%（1/81）. The results showed the differences in incidence of rotavirus infection among age groups, seasons and occupations were significant, but there were no gender and living place specific significant differences. The group A rotavirus genotype mainly included G9 P[8]（53.75%）, G2 P[4]（25.00%）, G3 P[8]（11.25%）, G1 P[8]（2.50%）,G4 P[6]（2.50%）. The study showed that G9 P[8] genotype was mainly G9-Ⅵsubtype according to the homology and evolutionary analysis of VP7 gene. The rotaviruses with same genotype were highly homogenous. Conclusion Rotavirus is a common pathogen causing diarrhea in Taizhou. Genotyping and evolution analysis showed the diversity and stability of group A rotavirus in this area. It is suggested to include G9 type rotavirus into vaccine strains, and conduct consecutive etiological surveillance in susceptible populations, especially young children.