难治性支原体肺炎患儿血清抗炎与致炎因子表达的变化研究

Changes of expression of serum antiinflammatory and proinflammatory cytokines in children with refractory Mycoplasma pneumoniae pneumonia

目的研究难治性肺炎支原体肺炎患儿血清炎症因子表达的变化情况,为难治性支原体感染肺炎患儿的防控与治疗提供参考依据。方法选取2015年10月-2017年6月医院收治的60例难治性肺炎支原体肺炎患儿为研究组,选取同时期的60例同龄健康儿童为对照组。分别比较两组研究对象及研究组中不同分类患儿血清炎症因子表达水平。结果研究组患儿血清抗炎因子白介素-4(IL-4)、白介素-10(IL-10)及转化生长因子-β(TGF-β)水平分别为(293.75±28.94)ng/L、(25.63±3.46)pg/ml及(236.76±24.98)pg/ml,血清致炎因子白介素-6(IL-6)、白介素-8(IL-8)及肿瘤坏死因子-α(TNF-α)水平分别为(7.88±0.93)pg/ml、(8.92±1.03)pg/ml及(1.82±0.31)ng/ml,均高于健康对照组儿童,差异有统计学意义(均P<0.05);研究组中不同分类患儿血清抗炎因子(IL-4、IL-10和TGF-β)及致炎因子(IL-6、IL-8和TNF-α)表达水平比较差异有统计学意义(P<0.05),其中进展性肺炎患儿水平最高。结论难治性肺炎支原体肺炎患儿血清抗炎与致炎因子均呈现高表达,且以进展性肺炎的表达更高,应重视对本类患儿进行上述抗炎与致炎因子表达的监测与干预。

OBJECTIVE To explore the changes of expression of serum inflammatory cytokines in children with refractory Mycoplasma pneumoniae pneumonia so as to provide guidance for prevention and treatment of refractory M.pneumoniae pneumonia in the children.METHODS Totally 60 children with refractory M.pneumoniae pneumonia who were treated in the hospital from Oct 2015 to Jun 2017 were assigned as the study group,meanwhile,60 healthy children who were as old as the children in the study group were chosen as the control group.The levels of serum inflammatory cytokines were observed and compared between the two groups of study objects and among the children in the study group.RESULTS The levels of serum anti-inflammatory cytokines interleukin-4（IL-4）,interleukin-10（IL-10）and transforming growth factor-β（TGF-β）of the study group were（293.75±28.94）ng/L,（25.63±3.46）pg/ml and（236.76±24.98）pg/ml,respectively;the levels of serum proinflammatory cytokines interleukin-6（IL-6）,interleukin-8（IL-8）and tumor necrosis factor-α（TNF-α）of the study group were（7.88±0.93）pg/ml,（8.92±1.03）pg/ml and（1.82±0.31）ng/ml,respectively;the levels of all the above indexes of the study group were significantly higher than those of the control group,and there were significant differences（all P〈0.05）.There were significant differences in the levels of IL-4,IL-10,TGF-β,IL-6,IL-8 and TNF-αamong the children in the study group（P〈0.05）,and the levels of the above indexes of the children with progressive pneumonia were the highest.CONCLUSIONThe children with refractory M.pneumoniae pneumonia show high expression of both serum anti-inflammatory and proinflammatory cytokines,the children with progressive pneumonia even higher.It is necessary to focus on the monitoring of the levels of serum anti-inflammatory and proinflammatory cytokine of the children and take intervention measures.