广西北部湾茅尾海红树林生境放线菌分离培养基的比较

A comparison of actinomycetes isolation medium with samples from mangrove habitats in Maowei Sea, Guangxi Beibu Gulf

【背景】红树林生境拥有丰富的微生物资源,分离获得更多的纯培养放线菌为新型抗生素的发现提供菌种资源。【目的】使用新型放线菌分离培养基,发掘广西北部湾茅尾海红树林生境放线菌资源,评估新型放线菌分离培养基分离效果。【方法】设计出新型放线菌分离培养基——椰子汁-海藻糖培养基,对广西北部湾茅尾海红树林根际土壤和红树林根皮放线菌进行分离。基于分子生物学鉴定方法,对获得的纯培养放线菌进行多样性分析。【结果】从红树林根际土壤样品中分离获得65株放线菌,分布在5个目10个科15个属,使用YIM171培养基分离到7个属,MA培养基分离到5个属,椰子汁-海藻糖培养基分离到11个属,其中包括4株潜在新种;从红树林根皮样品中分离获得19株放线菌,分布在4个目7个科10个属,使用YIM171和椰子汁-海藻糖培养基分别分离到7个属,包括4株潜在新种;使用椰子汁-海藻糖培养基在红树林生境两类样品中获得3株潜在新种。【结论】广西北部湾茅尾海红树林根际土壤和根皮中放线菌多样性丰富,使用椰子汁-海藻糖培养基分离放线菌效果较佳,可用作放线菌的分离培养基。

[Background] Mangrove habitats are rich in microbial resources. Isolation of more pure culture actinomycetes can provide strain resources for the discovery of new antibiotics. [Objective] A new actinomycete isolation medium was used to explore the strain resources of actinomycetes in mangrove habitats of Maowei Sea in Guangxi Beibu Gulf. Separation effect of new actinomycetes isolated culture medium was evaluated. [Methods] Coconut juice-mycose medium was newly designed and used for isolating actinobacteria from mangrove rhizosphere soil and mangrove root bark in Guangxi Beibu Gulf, Maowei Sea. The diversity analysis of pure culture actinomycetes was carried out based on molecular biology method. [Results] 65 strains of actinomycetes, including 4 potential new taxa, were isolated from the rhizosphere soil of mangrove which distributed in 5 orders, 10 families and 15 genera. Seven, five and eleven genera were isolated by YIM171, MA and coconut juice-mycose medium respectively. 19 strains of actinomycetes, including 4 potential new taxa, isolated from the root bark of mangrove were distributed in 4 orders, 7 families and 10 genera, 7 genera were isolated by YIM171 medium and coconut juice-mycose medium, respectively. Three potential new taxa were obtained from two kinds of samples in mangrove habitats using Coconut juice-mycose medium. [Conclusion] Rhizosphere soil and root bark of mangrove in Guangxi Beibu Gulf, Maowei Sea present an extremely abundant habitats for the isolation of a significant diversity of actinomycetes. Coconut juice-mycose medium is effective in the isolation of actinomycetes thus can be considered as the isolation medium for actinomycetes.