绿色木霉菌合成金纳米颗粒的可能性及影响因素

The possibility and influence factors of biosynthesis gold nanoparticles in Trichoderma viride

【背景】金纳米颗粒(AuNPs)凭借其稳定性、抗氧化性能和生物相容性在许多领域有广泛应用。目前关于微生物合成金纳米颗粒的研究较少。【目的】对微生物合成金纳米颗粒的可能性以及影响因素进行探究,有利于揭示具体的合成机制,发现AuNPs的特性以及合成位置与菌丝和影响因素的关系。【方法】以绿色木霉菌(Trichoderma viride)菌株(GIM3.141)为菌种资源,通过目视检测法、紫外可见分光光度计、X射线衍射和透射电镜等手段分析合成AuNPs的特征。探讨细胞内生物合成金纳米颗粒(AuNPs)的可能性,研究生物量、初始金离子浓度、溶液pH等因素对细胞内合成AuNPs的影响。【结果】X射线衍射分析表明AuNPs以金纳米晶体形态存在。透射电镜分析表明AuNPs主要位于细胞壁膜间隙,一小部分附着在细胞壁上。紫外可见分光光度计分析表明,金纳米颗粒粒径随着生物量添加量和溶液pH的升高而变小,随着初始金离子浓度的升高而变大。【结论】非致病性真菌绿色木霉菌可以在细胞内合成AuNPs,其中包括伪球形、三角形、四边形和六边形等多种形状,粒径范围从几纳米到三百纳米,为大规模、低成本、无污染地生物合成纳米颗粒工艺提供了菌种资源。

[Background] Gold nanoparticles（AuNPs） have various applications in optics, electronics, biomedicines and catalysis due to their high stability, oxidation resistance and biocompatibility. The current research on microbial synthesis of gold nanoparticles is less. [Objective] We performed the intracellular biosynthesis of AuNPs with the biomass of Trichoderma viride. The effects of the biomass addition, initial gold ions concentration and solution pH were also evaluated. [Methods] We performed the intracellular biosynthesis of AuNPs using biomass of Trichoderma viride（GIM3.141）. By visual method, ultraviolet visible（UV-vis） spectrophotometer and X-ray diffraction（XRD） and transmission electron microscopy（TEM） and other means to analyze the characteristics of the synthesis of AuNPs. The possibility of biosynthesis of AuNPs was discussed. The effect of reaction conditions on the characterizations of the AuNPs was also evaluated. [Results] The particle size decreased with the increase of biomass addition and solution pH, and increased with the increase of initial gold ions concentration. Reaction conditions such as biomass addition, solution pH and initial gold ions concentrations exhibited great effect on the AuNPs location and size distribution. [Conclusion] On exposing the biomass of Trichoderma viride to aqueous solution of HAuCl4, AuNPs with various shapes including pseudo-spherical, triangular, quadrilateral, and hexagonal ranging from several nanometers to more than 300 nm were produced. For large scale, low cost, clean biosynthesis nanoparticles process provides the species resources.