恩拉霉素高产菌株选育的研究

Breeding of high yield strain of enramycin

目的进一步提高恩拉霉素发酵生产水平。方法对杀真菌链霉菌RN16-7进行ARTP(常压室温等离子体)等离子与庆大霉素复合处理。结果经大量筛选,得到了恩拉霉素高产突变株RN16-242,其遗传性状稳定,发酵效价达7423u/mL,比出发菌株RN16-7(4703u/mL)提高了57.8%。菌株保存,采用甘油管低温保藏法、沙土管保藏法和冷冻真空干燥保藏法进行了比较,结果是甘油管低温保藏法最好。

Objective In order to improve the productive ferment level of the enramycin. Methods In this research, the original strain RN16-7 of Streptomyces fungicidicus was mutated by the combination of ARTP and gentamicin. Results The mutant RN16-242 was obtained with a high enramycin yield at 7423u/mL, which was up to 57.8% from the original RN16-7 （4703u/mL） and also of good stability. This article used methods of microbiological preservation： Glycerol cryopreservation, carrier preservation and freeze-drying/vacuum-drying. The results showed that the glycerol cryopreservation was best way.