黄芪对梗阻性黄疸肝缺血再灌注大鼠肾损伤的影响

Effect of Astragalus membranaceus on renal injury in rats with obstructive jaundice liver ischemia reperfusion

目的:探讨黄芪对梗阻性黄疸术后肝缺血再灌注肾脏功能损伤的影响。方法:选取60只大鼠,分为梗阻性黄疸组(A组)、梗阻性黄疸肝脏缺血再灌注组(B组)、梗阻性黄疸肝脏缺血再灌注+黄芪注射液组(C组)3组,每组20只,组内依据灌注时间点0、1、3、6、12 h分为5个亚组,每组4只。按时间点检测血清中尿素氮(BUN)、肌酐(Cr)、肿瘤坏死因子-α(TNF-α)的水平及肾脏组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)含量。结果:与A组相同时间点比,其余两组BUN、Cr和TNF-α水平升高(P<0.05);MDA、MPO含量升高,SOD含量减少(P<0.05);C组血清和肾脏组织中各项指标的改变程度均明显低于B组(P<0.05)。结论:黄芪能够保护梗阻性黄疸术后肝脏缺血再灌注引起的远端肾脏功能损伤,其与提高机体抗氧化能力,减少中性粒细胞聚集率、降低脂质过氧化及炎症反应相关。

Objective： To investigate the effect of Astragalus membranaceus on renal dysfunction after hepatic ischemia-reperfusion in obstructive jaundice. Methods： 60 rats were selected to be divided into three groups： obstructive jaundice（A）, obstructive jaundice liver ischemi a-reperfusion （B）, obstructive jaundice hepatic ischemia-reperfusion and astragalus injection（C）, 20 rats in each group, each group according to the reperfusion time 0, 1, 3, 6, 12 h divided into 5 subgroups,4 rats in each subgroup. Detection of serum levels of BUN, Cr and TNF-α in rat serum, the contents of MDA, SOD and MPO in kidney tissue. Results： Compare with A, the levels of BUN, Cr and TNF-α in the other two groups were increased; the contents of MDA and MPO were increased and the content of SOD was decreased（P〈0.05）, and the changes of indexes in C were significantly lower in B（P〈0.05）. Conclusion： Astragalus membranaceus can protect distal renal function damage caused by postoperative ischemia-reperfusion of obstructive jaundice, reducing neutrophil aggregation rate, reducing lipid peroxidation and inflammatory reaction.