血清miR-21和miR-423-5p对心脏瓣膜病伴有心力衰竭患者的诊断价值

Diagnostic values of miR-21 and miR-423-5p in patients with valvular heart disease and heart failure

目的探讨血清miR-21和miR-423-5p对心脏瓣膜病(VHD)引起心力衰竭的潜在诊断价值。方法选择2014年8月～2016年4月上海交通大学医学院附属第九人民医院心内科收治的伴心力衰竭的VHD患者60例为VHD组,选择同期正常人60名为对照组。通过实时荧光定量PCR(qRT-PCR)检测VHD伴有心力衰竭患者和正常人的血清miR-21和miR-423-5p表达,并利用ROC曲线分析miR-21和miR-423-5p作为VHD伴有心力衰竭诊断和预后生物标志物的临床价值。结果 VHD组患者血清miR-21和miR-423-5p表达明显高于对照组,差异有统计学意义(P <0.05)。伴有肺动脉高压(PH)的VHD患者血清miR-423-5p水平高于PH的VHD患者,差异有统计学意义(P <0.05)。ROC曲线结果显示,血清miR-21和miR-423-5p可以作为VHD伴心力衰竭的诊断标志物。结论血清miR-21和miR-423-5p具备诊断VHD伴有心力衰竭的临床价值。血清miR-423-5p可作为VHD伴有心力衰竭的诊断标志物。

Objective To investigate the potential diagnostic value of serum miR-21 and miR-423-5p for heart failure （HF） due to heart valve disease （VHD）. Methods From August 2014 to April 2016, in Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine, 60 VHD patients with HF were selected as VHD group, at the same period, 60 healthy persons were selected as control group. The levels of serum miR-21 and miR-423-5p were measured by qRT-PCR. The ROC curve was used analyse the clinical Value of miR-21 and miR-423-5p as VHD with biomarkers for diagnosis and prognosis of heart failure. Results The levels of miR-21 and miR-423-5p in sera of patients in VHD group were higher than control group, the differences were statistically significant （P 〈 0.05）. The levels of miR-423-5p in sera of VHD patients with pulmonary hypertension （PH） were higher than VHD patients without PH, the differences were statistically significant （P 〈 0.05）. ROC curve result showed that serum miR-21 and miR-423-5p had potential diagnostic values on HF due to VHD. Conclusion Serum miR-21 and miR-423-5p show potential diagnostic values on HF due to VHD. Moreover, serum miR-423-5p can be an effective biomarker for diagnosing VHD patients with PH.