摘要
液相色谱与质谱联用系统(LC-MS)在蛋白组学研究中发挥着重要作用,是目前生物学研究的热点。通过探索将大型液相色谱质谱联用系统应用到研究生实验教学的方法,建立了人类全蛋白的检测鉴定实验教学方法。使学生了解目前生命科学领域研究热点与研究方法,开阔学生专业视野,提高研究生对科研的积极性。
Liquid chromatography and mass spectrometry （LC-MS） plays an important role in proteomics research, meanwhile it is a hot spot in current biology. This paper explores the experimental teaching methods of application of large liquid chromatography mass spectrometry system, and establishment of analyzing method for the human protein. The experiment can make the students understand the hotspot of life science and research methods, and broaden students’ horizons and improve their enthusiasm of scientific research.
作者
万建
胡原
秦丽玮
熊国梅
李兵
万翠红
杨继红
WAN Jian;HU Yuan;QIN Liwei;XIONG Guomei;LI bing;WAN Cuihong;YANG Jihong(College of life Science,Modal Experiment Teaching Center of Hubei for Biolog;National Biology VirtualSimulation Experiment Teaching Center,Central China Normal University,Wuhan 430079,China)
出处
《实验室研究与探索》
CAS
北大核心
2018年第11期158-160,181,共4页
Research and Exploration In Laboratory
关键词
高分辨质谱
实验教学
大型精密仪器
人类全蛋白
检测鉴定
high resolution mass spectrometry
experimental teaching
large-scale precision instruments
human protein
detection
