两种大鼠阿尔茨海默病模型行为学实验的对比性研究

Praxiology experiment of two Alzheimer's disease models of rats:A comparative study

基于Aβ25-35(β-淀粉样蛋白)脑内注射和铝中毒两种AD动物模型进行行为学比较,除正常对照组外,其余各组大鼠腹腔注射D-半乳糖6周造成亚急性衰老模型,其中Aβ25-35模型组自第4周给予脑内海马定点注射Aβ25-35(1μg/μL)4μL,连续2 w;铝中毒模型组在腹腔注射D-半乳糖的同时灌胃给予三氯化铝(50 mg/kg·d)。通过Morris水迷宫及被动回避性跳台反射试验检测大鼠学习和记忆能力。结果显示,第4、5、6 d大鼠逃避潜伏期、第7 d穿台次数、跳台次数、错误次数、反射记忆能力潜伏期行为学检测指标,Aβ25-35模型组和铝中毒模型组与D-半乳糖组比较分别都具有显著性差异;而两种AD复合模型组上述行为学指标无显著性差异。结论:实验结果表明Aβ25-35脑内注射法和胃饲三氯化铝建立的AD动物模型在行为学上无统计学差异。

Two kinds of AD complex models of rats were established by Aβ 25-35 cerebral injection and aluminum composite poisoning. The praxiology experiment on the rats was conducted. Except the control group, rats in some groups were injected D-Gal constantly for six weeks by which the subacute decrepitude model was built. The rats in Aβ 25-35 model group was injected 1μg/μL 4μL Aβ 25-35 for two weeks to hippocampus and the ones in aluminum-poisoning model group were irrigated 50 mg/（kg·d） Alcl3 to stomach. The rat＇s abilities of study and memory were measured with Morris water maze test and jump platform experiment. The results showed that, compared with the D-gal group, the rat＇s ability of memory in Aβ 25-35 model group and aluminum-poisoning group descended obviously, but their behavior indicators had no significant difference. It is concluded that the AD models built by Aβ 25-35 model group and aluminum-poisoning have no statistical difference.