病毒巨噬细胞炎性蛋白ⅡN-端肽对乳腺癌miRNAs表达谱的影响

Effect of virus macrophage inflammatory protein Ⅱ N-terminal peptide on the expression spectrum of miRNAs in breast cancer

目的:阐明病毒巨噬细胞炎性蛋白Ⅱ(virus macrophage inflammatory protein-Ⅱ,v MIP-Ⅱ) N末端21个氨基酸的多肽(NT21MP)对乳腺癌MCF-7细胞miRNAs表达谱的调控作用。方法:收集各组细胞抽提RNA,RNA质量评估后逆转录成c DNA。采用染料法(SYBR GreenⅠ)进行相对定量分析,实验按照2-△△Ct解析法进行设计。结果:总共检测了168个miRNAs,以上调> 2倍或下调<0. 5倍筛选。相对于S组,N组升高的有9个:miR-223、miR-451a、miR-128、miR-489、miR-141、miR-320a、miR-155-3p、miR-335、miR-320e,降低的有2个:miR-15a、miR-339。结论:筛选得到NT21MP调控miRNAs差异表达谱,其为研究miRNAs在NT21MP调控中的作用机制提供依据。

Objective： To investigate the effects of virus macrophage inflammatory protein-Ⅱ（ v MIP-Ⅱ） N-terminal 21 amino acids peptide（ NT21 MP） on the expression profiles of miRNAs in breast cancer MCF-7 cell. Methods： The RNA in each group cells was extracted,and transcribed into c DNA. The experiment was designed according to the 2^-△△Ctanalytical method,and the miRNA expression profile was analyzed using the quantitative analysis of SYBR Green I. Results： A total of 168 miRNAs genes were detected,and the genes of expression upregulating two-fold or downregulating 0. 5-fold were screened. Compared with the S group,the expression levels of miR-223-3 p,miR-451 a,miR-128,miR-489,miR-141-3 p,miR-320 a,miR-155-3 p,miR-335-3 p and miR-320 e increased,and the expression levels of miR-15 a-5 p and miR-339-5 p decreased in N group. Conclusions： The differential expression profiles of miRNAs regulated by NT21 MP were screened to provide a basis for studyiny the mechanism of miRNAs in th regulation of NT21 MP.