血清肿瘤标志物CA153,CA125,CEA和HER-2联合检测在乳腺癌诊断中的价值

Value of Combined Detection of Tumor Markers CA153,CA125,CEA and HER-2 in the Diagnosis of Breast Cancer

目的探究血清肿瘤标志物癌抗原153(CA153)、糖链型抗原125(CA125)、癌胚抗原(CEA)和人类表皮生长因子受体-2(HER-2)联合检测在乳腺癌诊断中的价值。方法选取2017年1～12月期间收治的乳腺癌患者80例,同期乳腺良性疾病患者80例及同期健康体检者80例作为研究对象,分别作为乳腺癌组、乳腺良性疾病组及健康对照组,采用罗氏E411全自动化学发光免疫分析仪及配套试剂,定量检测全部入选研究对象的血清肿瘤标志物CA153,CA125,CEA和HER-2浓度,分析联合检测的意义。结果乳腺癌组的CA153,CA125,CEA和HER-2水平均显著高于乳腺良性疾病组和对照组,差异均有统计学意义(F=6.541～9.854,均P<0.05);乳腺良性疾病组的CA153,CA125,CEA和HER-2水平均高于对照组,差异均有统计学意义(F=4.953～7.924,P<0.05)。单纯检测CA153,CA125,CEA的敏感度对比,差异无统计学意义(χ~2=0.564,P>0.05)。单纯检测CA153,CA125,HER-2的特异度对比,差异无统计学意义(χ~2=0.405,P>0.05)。联合四者检测特异度及敏感度明显高于单项指标的特异度及敏感度,差异均有统计学意义(χ~2=0.472～0.913,P<0.05)。结论血清肿瘤标志物CA153,CA125,CEA和HER-2联合检测弥补了单一指标检测的不足,在乳腺癌诊断中敏感度高,值得临床推广。

Objective To explore the value of combined detection of serum tumor markers,such as carcinogen 153 （CA153）, carbohydrate chain antigen 125 （CA125）,carcinoembryonic antigen （CEA） and human epidermal growth factor receptor-2 （HER-2） ,in the diagnosis of breast cancer. Methods From January 2017 to December 2017,80 cases of breast cancer, 80 cases of benign breast disease and 80 cases of health check-up were selected as the subjects. They were divided into breast cancer group, benign breast disease group and healthy control group. Roche E411 automatic chemiluminescent immunoassay and related reagents were used to quantify the results. The concentrations of serum tumor markers CA153, CA125, CEA and HER-2 were detected in all subjects,and the significance of combined detection was analyzed. Results The levels of CA153, CA125, CEA and HER-2 in breast cancer group were significantly higher than those in the benign breast disease group and the control group,and the difference was statistically significant （t =6. 541-9. 854, all P〈0.05）. The levels of CA153, CA125, CEA and HER-2 in the benign breast disease group were higher than those in the control group,and the differences were of the significance （F= 4. 953-7. 924, P〈0.05）. There was no significant difference in sensitivity between CA153, CA125 and CEA （X2= 0. 564, P〉0.05）. Simple detection of CA153, CA125 and HER-2 specificity comparison, no significant difference （x2= 0. 405, P〉0.05）. The specificity and sensitivity of combined four methods were significantly higher than that of single method （x2= 0. 472-0. 913, P〈0.05）. Conclusion The combined detection of tumor markers CA153, CA125, CEA and HER-2 has high sensitivity in the diagnosis of breast cancer,and is worth popularizing.