二黄新伤凝胶膏治疗大鼠急性软组织损伤的效果研究

Effect of Erhuang Xinshang Gel on Acute Injury of Soft Tissue in Rats

目的:研究二黄新伤凝胶膏对急性软组织损伤疗效及作用机制。方法:"定量化重力打击法"造SD大鼠急性闭合性软组织损伤模型后,分别用二黄新伤凝胶膏(C组)、二黄新伤止痛软膏(D组)和云南白药膏(E组)治疗。进行临床评分及组织病理学观察;检测用药第1,4,7天,大鼠血清IL-1β和bFGF含量及对应mRNA在损伤部位新生肌肉中的表达。结果:二黄新伤凝胶膏可有效修复造模后的组织损伤;用药第4天,C,D及E组大鼠血清IL-1β含量及C和E组肌肉IL-1βmRNA表达水平均明显低于模型组,差异有统计学意义(P<0.05),C,D及E组的大鼠血清bFGF含量及C和E组bFGF mRNA表达水平明显高于模型组,差异有统计学意义(P<0.05)。结论:二黄新伤凝胶膏能修复大鼠急性软组织损伤,改善炎性因子和损伤修复因子。

Objective：To investigate the therapeutic effect and mechanism of Erhuang Xinshang gel in the treatment of a- cute injury of soft tissue. Methods.. The closed acute injury of soft tissue model of SD rats was established by ＂quantitative gravity strike method＂ and then treated with Erhuang Xinshang gel （Group C）, Erhuang Xinshang Zhitong ointment （Group D） and Yunnan Baiyao cream （Group E）, respectively. The wound healing process and histopathology were moni- tored and the levels of IL-1β and bFGF in serum were detected by ELISA method, and the expression of IL-1β and bFGF mRNA in tissues were detected by RT-PCR method. Results： Clinical and histopathological observation showed that Er- huang Xinshang gel was effective on tissue repairing. On the 4th day of treatment, the serum IL-1β levels in group C, D and E group and IL-1β mRNA expression in group C and E group were significantly lower than those in model group （P 〈0.05）, and the serum bFGF levels in group C, D and E group and the expression of bFGF mRNA in group C and E group were significantly higher than those in model group （P〈0. 05）. Conclusion.. Erhuang Xinshang gel can repair acute injury of soft tissue, improve inflammatory and damage repair factors in rats.