双着丝粒染色体自动分析生物剂量估算影响因素研究

Study on the influence factors of automatic analysis of dicentric chromosomes for biodosimetry

目的研究不同实验条件对双着丝粒染色体自动生物剂量估算结果的影响。方法不同方法制备染色体标本,遗传工作站自动分析双着丝粒畸变后,用本实验室拟合的"双着丝粒染色体自动分析剂量-效应曲线"进行生物剂量估算。结果受照血液20℃室温放置50 h、滴片细胞悬液浓度、中期分裂相分散度、染色深浅、重复扫描、遗传工作站扫描灵敏度设置、玻片细胞采集部位均不影响自动生物剂量估算结果(P>0.05)。结论双着丝粒染色体自动分析对实验中的一般性影响因素不敏感,有利于该方法在不同实验室间的推广。

Objective To study the influences of different experimental conditions on the results of automatic analysis of dicentric chromosomes for biodosimetry. Methods The dose effect curve of＂automatic analysis of dicentric chromosomes＂fitted by our laboratory to estimate the dose of samples prepared in different conditions after the genetic workstation automatically analyzed the dicentric chromesomes. Results The results of biodosimetry are not affected by the conditions of 50 hours at 20℃ of blood placement before inoculation,different cell suspensions concentration,different degree of dispersion in the metaphases,Wright-Giemsa staining depth,repeated scanning times,sensitivity parameter of genetic workstation and distribution positions of the metaphases on the slide（ P〉0.05 and have no significant difference）. Conclusion Automatic analysis of dicentric chromosomes is not sensitive to the general influence factors in the experiments,and is conducive to the promotion among different laboratories.