增殖抑制基因在宫颈癌组织中的功能作用

Function of hyperplasia suppressor gene in cervical tissues

目的探讨在宫颈癌组织中增殖抑制基因(HSG/Mfn2)的表达及意义,为宫颈癌的早期诊断和预后推测提供理论依据。方法收集2010年7月-2015年7月该院的临床标本,采用原位杂交和免疫组织化学的方法检测HSG/Mfn2在宫颈癌、宫颈上皮内瘤变(CIN)和正常宫颈组织中的表达。结果 HSG mRNA及其蛋白在宫颈癌标本中的阳性表达率分别为18.06%、20.83%;CINⅠ级中阳性表达率为48.15%、44.44%,CINⅡ级阳性表达率为40.00%、30.00%,CINⅢ级阳性表达率为28.57%、28.57%;正常宫颈组织中阳性表达率为56.00%、60.00%;HSG mRNA及其蛋白的表达与宫颈癌临床分期和分化程度有关(P<0.05),而与患者年龄、病理类型及淋巴转移情况无关(均P>0.05)。结论 HSG在宫颈癌组织中低表达,提示其与宫颈癌的发生发展密切相关。

Objective To explore the expression and significance of hyperplasia suppressor gene(HSG/Mfn2)in cervical carcinoma,in order to provide theoretical basis for the early diagnosis and prognosis prediction of cervical carcinoma.MethodsClinical samples were collected from July 2010 to July 2015 in the hospital.Immunohistochemically and insitu hybridization technique were used to detect the expression of HSG/Mfn2 in cervical carcinoma,cervical intraepithelial neoplasia(CIN)and normal cervical tissues.Results The positive rate of HSG mRNA and protein were 18.06%and 20.83%in cervical cancer tissues,48.15%and 44.44%in CINⅠ,40.00%and30.00%in CINⅡ,28.75%and 28.75%in CINⅢ,56.00%and 60.00%in normal cervical tissues.The expression of HSG mRNA and protein correlated with clinical stage and histological differentiation(P<0.05),but were not associated with the age,pathology and lymphatic metastasis(P>0.05).Conclusion The expression level of HSG/Mfn2 in cervical carcinoma is low,which may play an important role in the occurrence and development of cervical carcinoma.