沙棘籽油提取工艺优化及抗氧化活性研究

Antioxidant activity of oil in seabuckthorn seed and optimization of its extraction process

目的 优化沙棘籽中沙棘籽油的提取工艺，并评价其抗氧化活性。方法 通过Design-expert响应面软件对沙棘油提取温度、提取时间、料液比和粒度大小进行优化，并通过其抗1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基反应评价抗氧化活性。结果 沙棘籽油的最佳工艺为：提取时间3 h，料液比1∶8，提取温度80 ℃，粒度大小30目左右，提取产率最高，为11.13%，最佳反应时间8 min，沙棘油抗氧化活性随浓度增加逐渐增强，当样品加入量为4.00 ml时，清除率为77.62%。结论 此方法简单可靠，提取率高，沙棘籽油具有明显的抗氧化活性。

Objective To optimize the process conditions of the oil extraction of seabuckthorn oil and to evaluate its antioxidant activity by anti-free radical action. Methods The extraction time and particle size of sea-buckseed oil were optimized by using the response surface software design-expert. Its antio xidant activity was studied through its anti-dpph free radical reaction. Results The best process of seabuckthorn seed oil was extracting time 3 h, material liquid than 1：8, extraction temperature 80 ℃, about 30 mesh size, the yield is highest at 11.13%. The optimum reaction time was 8 min in control, and with the increase of concentration, seabuckthorn oil antioxidant activity increased, when the addition amount of 4.00 ml sample, clearance rate as high as 77.62%. Conclusions This method is simple and reliable, the extraction rate is high, and the test results show that the oil has obvious anti-oxidative effect, which can be used as whitening and wrinkling products to delay the aging of human body.