桄榔子醇提物对急性腹膜炎模型小鼠的改善作用及机制研究

Study on the Improvement Effect and Mechanism of Ethanol Extract of Arenga pinnata on Acute Peritonitis Model Mice

目的:探讨桄榔子醇提物对急性腹膜炎模型小鼠的改善作用及机制。方法:将小鼠按体质量随机分为空白对照组(蒸馏水)、模型对照组(蒸馏水)、阳性对照组(洛索洛芬钠片0.023 g/kg)和桄榔子醇提物高、中、低剂量组(6.5、3.25、1.625 g/kg,以提取物量计),每组24只,灌胃给予相应药物,连续给药3 d。末次给药30 min后,除空白对照组外,其余各组小鼠均腹腔注射酵母多糖溶液复制急性腹膜炎模型。腹腔注射酵母多糖溶液4 h内,观察各组小鼠的活动情况、反应状态和排泄情况;造模后每组取12只小鼠,立即尾静脉注射伊文思蓝染色液,收集腹腔灌洗液,观察各组小鼠腹腔毛细血管的通透性;腹腔注射酵母多糖溶液4 h后每组取剩余小鼠计数腹腔灌洗液中白细胞总数,采用酶联免疫吸附法检测各组小鼠腹腔灌洗液中炎症因子[白细胞介素1β(IL-1β)、IL-6、IL-10、肿瘤坏死因子α(TNF-α)和髓过氧化物酶(MPO)]的含量。结果:与空白对照组比较,模型对照组小鼠出现明显的扭体、腹部凹陷、蜷缩和腹泻等现象,腹腔毛细血管通透性明显增加(P<0.01),腹腔灌洗液中白细胞总数和IL-1β、IL-6、IL-10、TNF-α、MPO含量均明显增加(P<0.01)。与模型对照组比较,阳性对照组和桄榔子醇提物高、中、低剂量组小鼠上述现象仅偶有发生,腹腔毛细血管通透性明显降低(P<0.01),腹腔灌洗液中白细胞总数和IL-1β、TNF-α含量均明显减少(P<0.05或P<0.01),IL-10含量明显增加(P<0.05或P<0.01),桄榔子醇提物高、中剂量组小鼠腹腔灌洗液中IL-6、MPO含量均明显减少(P<0.05或P<0.01),且上述指标变化程度均与桄榔子醇提物呈剂量依赖性。结论:桄榔子醇提物对小鼠急性腹膜炎具有改善作用,其作用机制可能与降低腹腔毛细血管通透性、抑制炎症细胞浸润和调节腹腔中炎症因子分泌有关。

OBJECTIVE：To investigate the improvement effect and mechanism of ethanol extract of Arenga pinnata on acute peritonitis model mice. METHODS：The mice were randomly divided into blank control group（distilled water）,model control group （distilled water）,positive control group （Loxoprofen sodium tablets 0.023 g/kg） and ethanol extract of A. pinnata highdose,medium-dose and low-dose groups（6.5,3.25,1.625 g/kg,by the amount of extract）according to body weight,with 24 mice in each group. They were given relevant medicine intragastrically for consecutive 3 d. 30 min after last administration,except for blank control group,other groups were given zymosan solution intraperitoneally to induce acute peritonitis model. The activity,response and excretion of mice were observed within 4 h after intraperitoneal injection of zymosan solution. 12 mice of each group were given Evans blue staining solution immediately after modeling. Peritoneal fluid was collected to observe the permeability of abdominal capillary in mice. Within 4 h after intraperitoneal injection of zymosan solution,total number of white blood cells in residual mice was counted in each group. ELISA assay was adopted to determine the contents of inflammatory factors （IL-1 β ,IL-6,IL-10,TNF-α ,MPO）. RESULTS：Compared with blank control group,model control group suffered from significant writhing,abdominal retraction,crouch and diarrhea;peritoneal capillary permeability increased significantly（P〈0.01）. The total number of leukocyte and the contents of IL-1 β,IL-6,IL-10, TNF-α and MPO in peritoneal fluid were increased significantly（P〈0.01）. Compared with model control group,above phenomena of mice occurred occasionally in positive control group, ethanol extract of A. pinnata high-dose,medium-dose and low-dose groups,and peritoneal capillary permeability decreased significantly （P〈0.01）. Total number of leukocyte and the contents of IL-1 β and TNF-α in peritoneal fluid were decreased significantly（P〈0.05 or P〈0.01）,while IL-10 content was increased significantly（P〈0.05 or P〈0.01）. The contents of IL-6 and MPO in peritoneal fluid were decreased significantly in ethanol extract of A. pinnata high-dose and medium-dose groups（P〈0.05 or P〈0.01）,in dose-dependent manner. CONCLUSIONS：Ethanol extract of A. pinnata has improvement effect on acute peritonitis mice,the mechanism of which may be associated with reducing the permeability of abdominal capillaries,inhibiting inflammatory cell infiltration and regulating the secretion of inflammatory factors in abdominal cavity.