巴子颗粒对雷公藤多苷诱导少弱精子症模型大鼠精子质量影响的实验研究

Bazi Granules improves sperm quality in rats with oligoasthenozoospermia induced by multi-glycosides of tripterygium wilfordii

目的:探讨巴子颗粒对雷公藤多苷(GTW)诱导少弱精症大鼠精子质量的影响和作用机制。方法:36只雄性SD大鼠,随机分为正常组、模型组、阳性药组、巴子颗粒低剂量组、巴子颗粒中剂量组、巴子颗粒高剂量组,每组6只。除正常组外,其他各组均灌服GTW [30 mg/(kg·d)],连续灌胃40d建立少弱精子症模型。从41 d开始,阳性药组以五子衍宗丸灌胃(1. 02 g/kg),巴子颗粒低、中、高组以人体等效剂量3(5.27 g/kg)、6(10.54 g/kg)、12(21. 08 g/kg)倍用量灌胃,正常组与模型组以灌蒸馏水灌胃,28 d后测定精子质量及睾丸组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:巴子颗粒干预后,高、中、低各组精子浓度分别为(1 050. 71±203. 71)、(1 370. 06±166. 01)、(1 302. 53±476. 51)×10~6/ml,与模型组[(617. 01±237. 08)×10~6/ml]比较显著提升(P <0. 05);精子活力分别为(0. 56±0. 24)%、(0. 73±0. 14)%、(0. 70±0. 23)%,与模型组[(0. 07±0. 05)%]比较显著提升(P <0. 05);精子平均路径速度(VAP)分别为(85. 71±30. 35)、(83. 83±10. 31)、(75. 06±19. 70)μm/s,曲线速度(VCL)分别为(101. 76±23. 28)、(119. 60±21. 22)、(102. 11±32. 89)μm/s,精子直线速度(VSL)分别为(62. 75±7. 63)、(67. 80±5. 05)、(64. 11±12. 03)μm/s,均显著高于模型组[(43. 45±38. 74)μm/s、(53. 63±47. 91)μm/s、(40. 18±36. 86)μm/s](P <0. 05)。巴子颗粒高、中、低剂量组睾丸组织SOD含量分别为(380. 23±75. 07)、(349. 53±97. 48)、(415. 07±72. 01) U/mg,与模型组[(304. 62±27. 17) U/mg]相比有上升趋势,其中低剂量组升高显著(P <0. 05);巴子颗粒高、中、低剂量组睾丸组织MDA含量分别为(0. 33±0. 16)、(0. 22±0. 05)、(0. 34±0. 22) nmol/mg,与模型组[(0. 73±0. 20) nmol/mg]相比显著下降(P <0. 05)。结论:巴子颗粒能明显提升少弱精子症大鼠精子质量,其机制可能与修复氧化应激损伤,增强抗氧化能力有关。

Objective： To investigate the effect of Bazi Granules on sperm quality in male rats with oligoasthenozoospermia（ OAS）induced by multi-glycosides of tripterygium wilfordii（ GTW）. Methods： Thirty-six SD male rats were randomly divided into six groups of equal number： normal control,OAS model control,Wuziyanzong Pills（ WYP）,and low-,medium-and high-dose Bazi. The OAS model was established in the rats except those of the normal control group by intragastrical delivery of GTW at 30 mg/kg/d for 40 days. From the 41 st day,the animals of the normal and OAS model control groups were fed with distilled water,those of the WYP group treated by gavage with WYP at 1. 02 g/kg/d,and those of the low-,medium-and high-dose Bazi groups intragastically given Bazi Granules 3（ 5. 27 g/kg）,6（ 10. 54 g/kg） and 12（ 21. 08 g/kg） times,respectively,that of the human-equivalent dose. Semen parameters and the contents of superoxide dismutase（ SOD） and malondialdehyde（ MDA） in the testis tissue were determined after 28 days of treatment. Results： After treatment,the rats of the of the high-,medium-and low-dose Bazi groups,compared with the OAS model controls,showed significant increases in sperm concentration（ [1 050. 71 ± 203. 71],[1 370. 06 ± 166. 01]and [1 302. 53± 476. 51] vs [617. 01 ± 237. 08] ×10^6/ml,P〈0. 05）,sperm motility（ [0. 56 ± 0. 24]%,[0. 73 ± 0. 14]% and [0. 70 ±0. 23]% vs [0. 07 ± 0. 05]%,P〈0. 05）,sperm average path velocity（ [85. 71 ± 30. 35],[83. 83 ± 10. 31]and [75. 06 ±19. 70] vs [43. 45 ± 38. 74] μm/s,P〈0. 05）,sperm curvilinear velocity（ [101. 76 ± 23. 28],[119. 60 ± 21. 22] and[102. 11 ± 32. 89]vs [53. 63 ± 47. 91]μm/s,P〈0. 05）,sperm straight line velocity（ [62. 75 ± 7. 63],[67. 80 ± 5. 05]and [64. 11 ± 12. 03] vs [40. 18 ± 36. 86] μm/s,P〈0. 05）,and the SOD level（ [380. 23 ± 75. 07],[349. 53 ± 97. 48]and [415. 07 ± 72. 01]vs [304. 62 ± 27. 17]U/mg,P〈0. 05）,but a remarkable decrease in the MDA level（ [0. 33 ± 0. 16],[0. 22 ± 0. 05]and [0. 34 ± 0. 22]vs [0. 73 ± 0. 20]nmol/mg,P〈0. 05）. Conclusion： Bazi Granules can significantly improve the sperm quality of OAS rats,which may be related to its abilities of repairing oxidative stress injury and enhancing oxidation resistance.