基于网络药理学的当归补血汤治疗贫血的作用机制研究

Network Pharmacology-based Study on Mechanism of DangGuiBuXue Decoction in Treating Anemia

目的:利用网络药理学技术预测当归补血汤治疗贫血的作用机制。方法:通过TCMSP和TDT获取当归补血汤相关活性成分的化学结构;OMIM筛选贫血相关靶点;PPI构建当归补血汤和贫血的交互靶点;利用Cytoscape软件构建"化学成分-靶点-疾病"交互网络图。通过网络拓扑特征评价筛选出与当归补血汤治疗贫血方面的相关靶点;利用Bluego插件进行GO(基因功能)分析,并利用DAVID数据库对入选靶点进行相关通路富集。结果:筛选出当归补血汤治疗贫血的15个蛋白靶点;通过调节细胞循环、P53信号通路等11条通路来发挥治疗贫血的作用。结论:通过网络药理学预测当归补血汤中黄芪和当归配伍治疗贫血的作用机制,为其活性成分研究和临床应用提供理论依据。

Objective： To predict the mechanism of DangGuiBuXue decoction in treating anemia by using network pharmacology technique. Methods： TDT and TCMSP databases were used to screen the chemical composition of DangGuiBuXue decoction; OMIM database screened the targets of anemia; PPI database established the interaction targets between the drug and the disease. Cytoscape software was applied to construct the ＂compound-target-disease＂ complex network diagram. Targets of DangGuiBuXue decoction were selected, which played an important role in treating anemia by using the network topological parameters such as Degree, Betweenness Centrality, Closeness Centrality. In the ＂component-target-disease＂ network of DangGuiBuXue decoction, the targets which achieved the standard were enriched by the DAVID database. Results： 15 targets con＇elated with anemia were screened. DangGuiBuXue decoction was useful for treating anemia via regulating cell cycle arrest and eleven pathways such as Cell cycle, P53 signaling pathway, etc. Conclusion： The mechanism of DangGuiBuXue decoction in the treatment of anemia has been predicted by network pharmacology, which can provide the theoretical basis for its clinical application.