束缚应激致福利损伤小鼠肝脏中钙调蛋白的表达

The Study of Expression of CaM in Mice Liver after Welfare Impaired by Restrain Stress

目的研究束缚应激对小鼠肝脏内钙调蛋白表达的影响。方法选取3周龄ICR小鼠20只,随机分为对照组和束缚应激组,记录实验期间的个体增重和饲料消耗,束缚结束采血测定与福利相关的免疫和神经内分泌指标,及主要肝功能指标,采用Western Bolt法检测小鼠肝脏内钙调蛋白(CaM)和钙-钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)的蛋白表达水平。结果与对照组相比,束缚组小鼠的体增重和饲料能耗比(FER)显著降低(P<0.01),脾脏和胸腺的脏器指数显著降低(P<0.01,P<0.05),血清中皮质酮(CORT)和促肾上腺皮质激素(ACTH)以及主要肝功能指标谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)均显著升高(P<0.01,P<0.05),肝脏中CaM和CaMKⅡ的相对表达显著上升(P<0.05)。结论束缚应激致实验小鼠福利受损时,CaM和CaMKⅡ参与了小鼠福利损伤过程。

Objective To explore the effect of restraint stress on the expression of calmodulin in mice liver. Method Twenty three-week-old ICR rats were divided into two groups： control and rats under restraint stress. Individual weight gain and feed consumption were recorded during the experiment. After one week of continuous restraint treatment, blood sampling were taken to measure the neuroendocrine, immunology indexes and liver function indexes, the expression of CaM and CaMKⅡ in liver were evaluated by Western Bolt. Result Compared with the control group, the individual weight gain and FER of restraint group were decreased（P〈0.01）, the organ index of spleen and thymus were significantly decreased（P〈0.01,P〈0.05）, CORT, ACTH and liver function indexes AST, ALT, ALP were significantly increased（P〈0.01,P〈0.05）. The protein expression of CaM and CaMKⅡ were much higher than those in the control group（P〈0.05）. Conclusion After welfare damaged by restraint stress, CaM and CaMKⅡ were involved in the process of welfare being impaired.