摘要
黑龙江省农业科学院大豆研究所根据育种目标构建具有大豆病毒病、灰斑病、胞囊线虫病目标基因的抗性F2分离群体:黑农41(感SMV3)×哈91R3-301(抗SMV3)、黑农39(感FLS)×东农9674(抗FLS)、黑农33(感SCN)×灰布支黑豆(抗SCN)。针对不同病害,筛选与目的基因紧密连锁的SSR标记,定位QTL。利用已有标记在黑农51的回交转育后代群体中直接针对抗病基因进行精准选择,聚合目标基因,创造多抗高产大豆新品种黑农84。凝练技术方法建立多基因聚合的大豆育种体系,为大豆分子设计育种提供有益参考。
To the breeding objective, the Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences con- structed F2 isolation population with target genes of soybean virus disease, gray spot disease and cyst nematode disease : Hei- nong 41 (susceptible to SMV3 ) × Ha91R3-301 ( susceptible to SMV3 ), Heinong 39 ( susceptible to FLS), * Dongnong 9674 (resistant to SMV3), Heinong 33 (susceptible to SCN) x Black Soybean (resistant to SCN). For different diseases, we screened the SSR markers closely linked to the target gene and located the QTL. In order to create a new soybean variety Heinong 84 with multi-resistance and high yield, the target genes were aggregated by precise selection of disease-resistant genes in the backcross progeny population of Heinong 51. The technique of molecular marker gene polymerization was used to establish a polygenic soybean breeding system, which could provide a useful reference for molecular design breeding of soy- bean.
作者
栾晓燕
刘鑫磊
薛永国
马岩松
王家军
张必弦
于佰双
王广金
LUAN Xiao-yan;LIU Xin-lei;MA Yan-song;XUE Yong-guo;ZHANG Bi-xian;WANG Jia-jun;YU Bai-shuang;WANG Guang-jin(Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2018年第6期839-842,共4页
Soybean Science
基金
"十三五"国家重点研发计划课题(2017YFD0101306-01-01)
哈尔滨市科技创新人才项目(2015RQQYJ5058)
关键词
分子标记
基因聚合
大豆品种
黑农84
Molecular marker
Gene polymerization
Soybean cuhivars
Heinong 84