多抗高产大豆新品种黑农84的选育研究

Innovating Soybean Heinong 84 with High Quality and Multi-resistance by Molecular Marker Gene Polymerization

黑龙江省农业科学院大豆研究所根据育种目标构建具有大豆病毒病、灰斑病、胞囊线虫病目标基因的抗性F2分离群体:黑农41(感SMV3)×哈91R3-301(抗SMV3)、黑农39(感FLS)×东农9674(抗FLS)、黑农33(感SCN)×灰布支黑豆(抗SCN)。针对不同病害,筛选与目的基因紧密连锁的SSR标记,定位QTL。利用已有标记在黑农51的回交转育后代群体中直接针对抗病基因进行精准选择,聚合目标基因,创造多抗高产大豆新品种黑农84。凝练技术方法建立多基因聚合的大豆育种体系,为大豆分子设计育种提供有益参考。

To the breeding objective, the Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences con- structed F2 isolation population with target genes of soybean virus disease, gray spot disease and cyst nematode disease ： Hei- nong 41 （susceptible to SMV3 ） × Ha91R3-301 （ susceptible to SMV3 ）, Heinong 39 （ susceptible to FLS）, ＊ Dongnong 9674 （resistant to SMV3）, Heinong 33 （susceptible to SCN） x Black Soybean （resistant to SCN）. For different diseases, we screened the SSR markers closely linked to the target gene and located the QTL. In order to create a new soybean variety Heinong 84 with multi-resistance and high yield, the target genes were aggregated by precise selection of disease-resistant genes in the backcross progeny population of Heinong 51. The technique of molecular marker gene polymerization was used to establish a polygenic soybean breeding system, which could provide a useful reference for molecular design breeding of soy- bean.