龙葵碱对Jurkat细胞增殖、凋亡的作用及机制

Effect and mechanism of Solanine on proliferation and apoptosis of Jurkat cells

目的探讨龙葵碱对急性T淋巴细胞白血病（T－ALL）Jurkat细胞增殖、凋亡的作用及其机制。方法采用不同质量浓度龙葵碱分别处理Jurkat细胞24、48、72 h后，通过CCK－8法观察龙葵碱对Jurkat细胞增殖的影响，通过流式细胞术检测龙葵碱对Jurkat细胞凋亡的影响，不同浓度龙葵碱处理Jurkat细胞后，Western blot法检测Bcl－2和Bax的表达；并通过实时荧光定量聚合酶链反应（qPCR）技术检测Bcl－2 mRNA和Bax mRNA的表达水平。结果CCK－8结果显示：龙葵碱显著抑制Jurkat细胞增殖，并呈剂量和时间依赖性；流式细胞术检测凋亡结果显示：0、4、16 mg/L的龙葵碱处理Jurkat细胞24 h后，细胞的凋亡比例分别为（2.40±0.98）%、（28.43±4.86）%、（41.56±1.87）%，呈剂量依赖性。Western blot结果显示：龙葵碱在Jurkat细胞中能增加Bax表达，降低Bcl－2表达，均呈剂量依赖性。结论龙葵碱能显著抑制Jurkat细胞株的增殖，诱导其凋亡，其机制与上调Bax表达及下调Bcl－2表达有关。

ObjectiveTo explore the effect of proliferation and apoptosis of Solanine on acute T lymphocyte leukemia （T-ALL） Jurkat cells and its mechanism.MethodsAfter treated with different concentrations of Solanine, the proliferation of Jurkat cells was detected by CCK-8 assay, and the effect of Solanine on apoptosis of Jurkat cells were detected by flow cytometry.The expression of Bcl-2 and Bax in Jurkat cells were detected by Western blot, and the expression levels of Bcl-2 mRNA and Bax mRNA were detected by real-time fluorescence quantitative polyme-rase chain reaction.ResultsCCK-8 assay showed that Solanine significantly inhibited the proliferation of Jurkat cells in a dose-and time-dependent manner. The results of flow cytometry showed that the apoptosis rates of Jurkat cells treated with Solanine for 24 h were （2.40±0.98）%, （28.43±4.86）%, （41.56±1.87）%, respectively, in a dose-dependent manner. Western blot showed that Solanine could increase the expression of Bax and decrease the expression of Bcl-2 in Jurkat cells, and they all were dose-dependent.ConclusionSolanine can significantly inhibit the proliferation and induce apoptosis of Jurkat cells. The mechanism is related to the up-regulation of Bax expression and down-regulation of Bcl-2 expression.