卵母细胞人工激活技术在卵胞质单精子注射受精失败后的应用

Application of artifcial oocyte activation in fertilization failure of intracytoplasmic sperm injection

卵胞质内单精子注射(ICSI)受精失败是辅助生殖治疗中面临的一项难题,近年来卵母细胞人工激活(artificial oocyte activation,AOA)技术的应用使人们看到了解决这一问题的希望。但由于对卵母细胞激活失败的机制还缺乏明确的研究,AOA技术也并不能解决所有类型的ICSI受精失败,其临床应用指征和安全性仍然是人们关注的焦点。虽然目前的研究未显示出AOA技术会明显增加子代的出生缺陷,但该技术还是应该谨慎地用于有合适指征的病例。本综述从ICSI受精失败机理和类型,AOA的临床应用及其安全性等方面将该技术目前的研究进展予以总结,以期为生殖医学工作者提供较为全面的参考。

Fertilization failure is a serious challenge of assisted reproduction treatment especially for intracytoplasmic sperm injection （ICSI） cycles, in recent years, artificial oocyte activation （AOA） technology had shined a light on this problem. But the mechanism of oocyte activation failure is still a lack of clear research, as AOA cannot recover all types of fertilization failure, the clinical indications and safety of AOA is still the focus of attention. Although the current study did not show the AOA technology could significantly increase their offspring birth defects, this technology should be cautiously used for selected cases with appropriate indications. This article summarized recent progress of rnechanisms and types oflCSI fertilization failure, also the application of AOA and its safety, hoping to give a general view of AOA technique for the ART clinicians and embryologists.