吉林省2016年手足口病患儿肠道病毒71型VP1编码区基因分析

Genetic characteristics of VP1 encoding region gene of enterovirus 71 in children with hand,foot and mouth disease in Jilin Province,China in 2016

目的分析吉林省2016年肠道病毒71型(enterovirus 71,EV71)流行株基因学特征及其变异情况。方法采集2016年吉林省9个市、州送检的临床诊断为手足口病(hand foot and mouth disease,HFMD)298份标本,分离病毒获得阳性分离物,利用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)扩增阳性分离物VP1编码区,并对扩增产物进行核苷酸序列测定,应用Mega6和Bioedit 7. 01软件分析扩增序列基因亲缘关系和氨基酸位点变异。结果吉林省2016年分离的38株EV71同属于C4a基因亚型,并在VP1编码区共发生7处氨基酸位点的变异。结论吉林省2016年EV71流行株与C4a代表株位于同一分支属于C4a基因亚型,且发生了多位点氨基酸变异。

Objective To analyze the genetic characteristics and variation of enterovirus 71（EV71） epidemic in Jilin Province,China in 2016. Methods A total of 298 samples from patients with hand,foot and mouth disease in nine cities of Jilin Province in 2016 were collected,from which the positive isolates were obtained by virus isolation for amplification of VP1 encoding region gene by reverse transcription-polymerase chain reaction（RT-PCR）. The nucleotides of PCR products were sequenced,while the genetic relationship and variation of amino sequences were analyzed by using Mega6 and Bioedit 7. 01 software. Results The 38 EV71 isolates in Jilin Province in 2016 belonged to the C4 a subgenotype,and seven amino acid sites varied in the VP1 coding region. Conclusion The EV71 strains isolated in Jilin in 2016 were in the same branch as that of representative C4 a strain,which belonged C4 a gene subtype,with amino acid variants in several sites.