ACE I/D基因多态性及血清ACE活性与中国老年人骨丢失的关系

Relationship between angiotensin converting enzyme insertion/deletion polymorphism,serum angiotensin converting enzyme activity and bone loss in elder Chinese

目的探讨血管紧张素转化酶(angiotensin converting enzyme,ACE) I/D基因多态性以及血清ACE活性与中国老年人骨丢失的关系。方法采用问卷调查方式采集研究对象的人体测量学数据、生活方式、个人既往病史及用药史;利用双能X线骨密度仪测量骨密度;采集清晨空腹血分离血清及提取DNA,采用PCR分析ACE I/D基因多态性;血清ACE活性采用动力学试剂盒测定。利用4年随访时的骨密度和基线骨密度计算骨密度改变情况。采用多元线性回归分析方法分析ACE I/D基因多态性、血清ACE活性与骨丢失的关系。结果多元线性回归分析结果显示,男性(n=1 582)和女性(n=1 730)受访者ACE I/D基因多态性与骨丢失无关(男性脊柱、髋部、股骨颈的P值分别为0. 914,0. 345和0. 536,女性脊柱、髋部、股骨颈的P值分别为0. 711,0. 536和0. 650)。男性(n=1 119)和女性(n=1 067)受访者,血清血管紧张素转化酶活性与中国老年人的骨丢失无相关性(男性脊柱、髋部、股骨颈的P值分别为0. 698、0. 311和0. 548,女性脊柱、髋部、股骨颈的P值分别为0. 705,0. 915和0. 249)。结论 ACE I/D基因多态性及血清ACE活性与中国老年人的骨丢失没有相关性。

Objective To investigate the relationship between angiotensin converting enzyme （ACE） I/D polymorphism, serum ACE activity and bone loss in elder Chinese. Methods A standardized structured face-to-face interview was performed to collect demographic informations, life styles, personal medical history, and medication history. Bone mineral density （BMD） was measured using dual-energy X-ray absorptiometry （DXA）. Peripheral venous blood was taken after an overnight fasting for serum isolation and DNA extraction. I/D genotypes of ACE were determined by polymerase chain reaction （PCR） amplification. Serum ACE activity was determined photometrically by a commercially available kinetic kit. BMD changes were calculated by subtracting BMD at the baseline examination for BMD at the year 4 follow-up examination, and were expressed as the yearly percentage changes of the baseline value. Multiple linear regression analysis was used to examine the relationship between ACE I/D polymorphism, serum ACE activity and bone loss. Results Linear regression analysis results showed that there was no significant difference of yearly percentage bone loss was found between different ACE I/D genotypes both in men and women.In men, the P -value of spine, total hip, and femoral neck bone loss was 0.914, 0.345, and 0.536, respectively. In women, the P -value of spine, total hip, and femoral neck bone loss was 0.711, 0.536, and 0.650, respectively. Linear regression analysis showed that there was no significant difference of yearly percentage bone loss between different serum ACE activity quartile groups both in men and women. In men, the P -value of spine, total hip, and femoral neck bone loss was 0.698, 0.311 and 0.548 respectively. In women, the P -value of spine, total hip, and femoral neck bone loss was 0.705,0.915, and 0.249, respectively. Conclusion There was no relationship between ACE I/D polymorphism, serum ACE activity and bone loss in elder Chinese.