摘要
目的调查该地区女性人乳头瘤病毒(HPV)感染及其亚型的分布情况。方法采用实时荧光聚合酶链反应(PCR)技术,对2016年2月至2018年2月在该院妇科就诊的5 737例女性宫颈分泌物进行HPV检测并统计分析。结果 5 737例女性感染HPV的有1 105例,感染率为19.26%,其4个年龄组:≤30岁、>30~40岁、>40~50岁和>50岁女性HPV感染率分别为20.22%、19.09%、17.70%和21.53%,其中>40~50岁与>50岁年龄组女性HPV感染率差异有统计学意义(P<0.05)。1 105例HPV感染患者中,HPV单一感染的有802例(72.58%);多重感染的有303例(27.42%),以双重感染为主(219例)。1 105例HPV感染标本中共检测出HPV亚型1 537个(多重感染重复计数),其中高危型HPV共1 372例(89.26%),低危型165例(10.74%),HPV感染率排在前5位的基因型分别是HPV-52(3.68%)、HPV-16(2.96%)、HPV-53(2.75%)、HPV-58(2.63%)和HPV-81(1.83%)。结论六合地区女性感染较高的高危型HPV亚型依次为HPV-52、HPV-16、HPV-53和HPV-58型,低危型感染较多的是HPV-81型。HPV感染率随年龄增长呈"U"型变化。
Objective To investigate the incidence of human papillomavirus (HPV) infection and its subtype distribution among female human in the region. Methods Using real-time fluorescence PCR technique,HPV detection and statistical analysis were performed in 5 737 female cervical secretions from February 2016 to February 2018 in the department of gynecology,physical examination and ward of our hospital. Results Among the 5 737 female patients,1 105 cases were infected with HPV (19.26%).The rates of HPV infection in the four age groups(lower than 30 years,30-40 years,40-50 years and older than 50 years) were 20.22 %, 19.09 %,17.7% and 21.53%,respectively.HPV infection rates between the group of 40-50 years and older than 50 years group had statistical differences ( P 〈0.05).In 1 105 cases of HPV infection,there were 802 cases (72.58%) with HPV single infection,303 cases with multiple infection (27.42%) and of which,double infection was the main type (219 cases).A total of 1 537 subtypes of HPV (multiple infection repeat count) were detected in 1 105 cases of HPV infection,there were 1 372 cases (89.26%) with high-risk HPV,165 cases (10.74%) with low-risk HPV.The subtypes of HPV infection ranked in the top five were HPV-52 ( 3.68 %),HPV-16 (2.96%),HPV-53 (2.75%),HPV-58 (2.63%) and HPV-81 (1.83%). Conclusion In Liuhe region,the high-risk HPV infection of women are mainly HPV-52,HPV-16,HPV-53 and HPV-58,and the low-risk ones is HPV-81.The rate of HPV infection shows a "U" type change with age.
作者
陈小伟
胡利萍
阮福明
CHEN Xiaowei;HU Liping;RUAN Fuming(Department of Clinical Laboratory,Liuhe People′s Hospital,Nanjing,Jiangsu 211500,China)
出处
《检验医学与临床》
CAS
2018年第23期3549-3552,共4页
Laboratory Medicine and Clinic
关键词
人乳头瘤病毒
宫颈癌
基因检测
human papilloma virus
cervical cancer
gene detection