期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

全人源单克隆抗体制备技术的研究进展

下载PDF
导出
摘要 1975年,KOHLER等[1]通过杂交瘤技术成功获得了具有抗原特异性的鼠源性单克隆抗体,从而开启了单克隆抗体开发的新纪元。单克隆抗体具有专一性高、亲和力强等特点,已成为多种人类疾病诊断及治疗的重要工具。
作者 唐亚华 胡慧明 朱美玲
机构地区 南华大学附属南华医院药学部 南华大学附属南华医院内分泌科
出处 《现代医药卫生》 2018年第23期3642-3645,共4页 Journal of Modern Medicine & Health
关键词 全人源单克隆抗体 噬菌体技术 转基因小鼠技术 综述
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献5

二级参考文献22

  • 1Sullivan M, Kaur K, Pauli N, et al. Harnessing the immune system's arsenal: producing human monoclonal antibodies for therapeutics and investigating immune responses. F1000 Biol Rep, 2011, 3: 17.
  • 2Crowe JE Jr. Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches. Vaccine, 2009, 27(Suppl 6): G47-G51.
  • 3Morris L, Chen X, Alam M, et al. Isolation of a human anti-HIV gp41 membrane proximal region neutralizing antibody by antigen-specific single B cell sorting. PLoS One, 2011, 6(9): e23532.
  • 4Gilbert AE, Karagiannis P, Dodev T, et al. Monitoring the systemic human memory B cell compartment of melanoma patients for anti-tumor IgG antibodies. PLoS One, 2011, 6(4): e19330.
  • 5Menard L, Samuels J, Ng YS, et al. Inflammation-independent defective early B cell tolerance checkpoints in rheumatoid arthritis. Arth Rheum, 2011, 63(5): 1237-1245.
  • 6Jin A, Ozawa T, Tajiri K, et al. A rapid and efficient single-cell manipulation method for screening antigen-specific antibody-secreting cells from human peripheral blood. Nat Med, 2009, 15(9): 1088-1092.
  • 7Kuppers R, Zhao M, Hansmann ML, et al. Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections. EMBO J, 1993, 12(13): 4955-4967.
  • 8Kurosawa N, Yoshioka M, Isobe M. Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells. BMC Biotechnol, 2011, 11(1): 39.
  • 9Ozawa T, Kishi H, Muraguchi A. Amplification and analysis of cDNA generated from a single cell by 5'-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells. Biotechniques, 2006, 40(4): 469-470, 472, 474 passim.
  • 10Obiakor H, Sehgal D, Dasso JF, et al. A comparison of hydraulic and laser capture microdissection methods for collection of single B cells, PCR, and sequencing of antibody VDJ. Anal Biochem, 2002, 306(1): 55-62.

共引文献30

现代医药卫生
2018年 第23期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部