植物油中苯并(a)芘含量测定方法的建立

Establishment of method for determination of benzo( a) pyrene content in vegetable oil

采用先皂化再层析洗脱的方法对样品进行前处理,建立了高效液相色谱法测定植物油中苯并(a)芘含量的方法。结果表明:建立的方法加标回收率为99. 5%～106%,RSD为0. 68%～3. 6%,前处理具有成本低(<10元/样)、耗时短(60 min),试剂无水乙醇无毒、环保的优点,适用于大批量植物油中苯并(a)芘含量的测定。

A method for determination of benzene （a） pyrene content in vegetable oil by high performance liquid chromatography was established using sample pretreatment of saponification and chromatographic elution. The results showed that the standard addition recovery and RSD of this method were 99.5% - 106% and 0.68% -3.6% respectively, and pretreatment method had the advantages of low cost （less than ￥ 10 per sample） ,short time （60 min）, non -toxic and environmental protection （ethanol）, which was applicable to large quantities of vegetable oil in the determination of benzo（a） pyrene content.