UPLC-PDA-MS鉴定经大孔树脂纯化美藤果壳酚类物质

Identification of phenolic compounds from Sacha Inchi shells purified by macroporous resin using UPLC-PDA-MS

以经大孔树脂纯化的美藤果壳酚类物质为原料,用预制硅胶板薄层层析法将其粗分为4个条带,富集各个条带中的物质后使用UPLC-PDA-MS检测,确定其中的酚类物质为:1号条带中有香草酸己糖苷,1,2-芥子酰阿魏酸酰龙胆二糖(待定),矢车菊素3-(6″-阿魏酰槐糖苷)-5-葡糖苷,锦葵花色素3-葡萄糖苷,锦葵花色素3-葡萄糖苷二倍体; 2号条带中有香草酸己糖苷,香草酸己糖苷三倍体以及香草酸己糖苷二倍体; 3号条带中有对羟基苯甲酸,羟基苯甲酸己糖苷,对香豆酸己糖苷以及对香豆酸四倍体; 4号条带中有对羟基苯甲酸以及对香豆酸。

The phenolic compounds from Sacha Inchi shells purified by macroporous resin were divided into four bands by preparative thin layer chromatography. Then UPLC - PDA - MS was used to analyze the compounds in every band after enrichment. The structures of phenolic compounds were identified as follows ： vanillic acid hexoside, 1,2 - disinapoyl - feruloyl - gentiobiose （ undecided）, cyanidin 3 - （6＂-feruloylsophoroside） -5 -glucoside, malvidin 3 -glucoside and malvidin 3 -glucoside dimer in band 1 ; vanillic acid hexoside, vanillic acid hexoside trimer and vanillic acid hexoside dimer in band 2 ; p- hydroxybenzoic acid, hydroxybenzoic acid hexoside, p- coumaroyl hexose and p- coumaric acid tet- ramer in band 3 ; p - hydroxybenzoic acid and p - coumaric acid in band 4.