海洛因对仔鼠视网膜的氧化损伤和特异蛋白表达的影响

Effects of heroin on oxidative damage and specific protein expression in retina of newborn mice

目的探讨海洛因对小鼠视网膜的氧化损伤和特异蛋白表达的影响。方法出生7 d龄的昆明仔鼠60只,随机分为对照组和海洛因组。海洛因组用递增剂量(0.0571、0.0667、0.070 6 g/kg)腹腔连续(1～5 d、6～10 d和11～15 d)注射海洛因溶液15 d,每天两次(9∶00 Am,16∶00 Pm),每次0.2 ml,对照组注射等量生理盐水。用比色法检测仔鼠血浆乳酸脱氢酶(LDH)活性和谷胱甘肽(GSH)含量及视网膜组织中诱导型一氧化氮合酶(iNOS)活性的变化,用免疫组织化学法检测视网膜组织Caspase-3、核因子-κB(NF-κB)蛋白表达的变化。结果与对照组相比,海洛因组仔鼠血浆GSH含量显著降低,LDH活性显著上升,海洛因组仔鼠视网膜组织iNOS活性增加;海洛因组仔鼠视网膜组织Caspase-3、NF-κB蛋白的阳性表达量升高,平均吸光度值明显增大。结论海洛因影响动物血浆GSH含量和LDH活性及视网膜组织iNOS的活性,诱导视网膜组织Caspase-3、NF-κB蛋白的表达,影响动物视网膜组织的结构。

Objective To explore the effects of heroin on oxidative damage and specific protein expression in mice retina. Methods Sixty mice were randomly divided into 2 groups： the control group and the heroin group. The mice of heroin group were injected intraperitoneally with heroin of the increasing doses （0. 0571 g/kg, 0. 0667 g/kg, 0. 0706 g/ kg） continuous（ 1-5, 6-10 and 11-15 days） for 15 days（ twice a day） ,and the control group was injected with equivalence saline, activities of lactic dehydrogenase （LDH） and content of glutathione （ GSH ） in plasma, activities of inducible nitric oxide synthase （iNOS） in retinal tissue were detected by colorimetry. The expressions of Caspase-3 and nuclear factor-κB （NF-κB） protein of retina were examined by immunohistochemistry method . Results Compared with control group, the content of plasma GSH remarkable lower in heroin group. Meanwhile,the activities of plasma LDH remarkable rise in heroin group,the activities of mice retina iNOS remarkable increase in heroin group. The positive expressions of Caspase-3, NF-κB protein in retina were significantly increased. Conclusion Heroin may effect on GSH content and LDS activities in plasma and activity of iNOS in retinal tissue,induces the expressions of Caspase-3 and NF-κB protein of retinal cells,to influence the structure of the animal retina.