复方鹅绒藤凝胶膏剂抗炎镇痛作用研究

Anti-inflammatory and analgesic effects of Cynanchum sibiricum Willd extract

目的:探讨复方鹅绒藤凝胶膏剂(CSW)抗炎镇痛作用。方法:采用小鼠热板法和冰醋酸扭体法建立小鼠疼痛模型,考察复方鹅绒藤凝胶膏剂外用时的镇痛效果,采用二甲苯小鼠耳肿胀实验和慢性肉芽肿实验评价复方鹅绒藤凝胶膏剂的抗炎作用,利用酶联免疫分析试剂盒测定炎症部位PGE2含量。结果:复方鹅绒藤低、中、高三个剂量组均能显著减少醋酸致小鼠扭体次数(P <0. 05),在热板实验中,复方鹅绒藤低、中、高剂量组对提高小鼠痛阈差异无统计学意义(P <0. 05),复方鹅绒藤低、中、高三个剂量组能显著缓解二甲苯所致小鼠耳肿胀(P <0. 05),且抑制小鼠肉芽肿具有显著性(P <0. 05),复方鹅绒藤中、高剂量组与模型组比较,能显著降低炎症部位PGE2的含量。结论:复方鹅绒藤凝胶膏剂具有抗炎镇痛作用,尤其对炎症引起的疼痛具有较好的抑制效果且其抗炎镇痛机制与抑制炎症部位PGE2的产生和释放有关。

The aim of this study was to investigate the anti-inflammatory and analgesic effects of Cynanchum sibiricum willd extract. Hot plate test and acetic acid writhing test were used in researching the analgesic effects of Cynanchum sibiricum willd extract. The xylene-induced ear edema model and the chronic inflammatory model of granuloma induced by cotton pellet implantation were used in researching the inflammatory effects. Meanwhile, rat paw edema was used to study the PGE 2 level in inflammatory tissues. Compared with the normal saline group,high-middle-and low-dose（6.25 g/kg,12.50 g/kg and 25.00 g/kg） groups could significantly reduced the writhing number （P＜0.05） and inhibit ear edema in mice. Also, after treatment with high- and middle-dose, the pain threshold with hot plate method was significantly prolonged （P＜0.05）, the granuloma of rats was decreased （P＜0.05）. The results showed that compound velveteen gypsum gel paste has anti-inflammatory and analgesic effect, especially for inflammation caused by inflammation has a good inhibitory effect.