2-苯乙醇胁迫下酿酒酵母差异表达基因分析

Differential Expression of Saccharomyces cerevisiae Genes under the Stress of 2-Phenylethanol

2-苯乙醇(2-PE)是一种具有玫瑰花香气的高级芳香醇,广泛应用于香精香料的调配。微生物转化是生产天然2-PE最可行的方法,但其瓶颈问题是2-PE对细胞生理活性的抑制。2-PE对酿酒酵母毒性作用的分子机制还没有得到很好的研究。研究利用RNA-Seq高通量测序技术,测定分析2-PE胁迫下及其对照组的酿酒酵母转录组。结果显示,2-PE引起了酿酒酵母中1 853个基因的差异表达,其中1 122个基因表达上调,731个基因表达下调。这些基因的GO和KEGG富集分析显示,编码线粒体蛋白、细胞质蛋白和质膜蛋白的大多数基因显著上调,而与氨基酸代谢有关的酶则下调。这些结果表明,2-PE抑制了质膜蛋白的合成,抑制了生长所需的养分的运输。研究的结果可为2-PE对酵母及其他微生物抑制的机制研究提供依据。

2-phenylethanol （2-PE） is a kind of advanced aromatic alcohol with rose fragrance,which is wildly used for the deployment of flavors and fragrances. The bottleneck problem of microbial transformation, the most feasible method for the production of natural 2-PE, is the inhibition of 2-PE to cell viability. The molecular mechanisms of the inhibition effect of 2-PE to Saccharomyces cerevisi- ae is not still well-studied. In this study, we analyzed the transcriptomes of S cerevisiae under the stress of 2-PE and its control group, respectively, using RNA-Seq technology. We identified 1 853 differentially expressed genes of S cerevisiae between two groups, in which 1 122 genes were up-regulated and 731 genes were down-regulated. GO and KEGG enrichment analyses of these genes suggested that most genes encoding mitochondrial proteins, cytoplasmic and plasma membrane proteins were significantly up-regulated,whereas the enzymes related to amino acid metabolism were down-regula- ted. These results indicated that 2-PE suppressed the synthesis of plasma membrane proteins,which suppressed the transport of nutrients required for growth. The findings in this study will provide in- sight into the inhibitory mechanism of 2-PE to yeast and other microbes.