肠复康方对大肠癌细胞多药耐药的逆转作用机制研究

目的:通过血清药理学(serum pharmacology)及分子生物学(molecular biology)原理探讨肠复康方对大肠多药耐药逆转机理。方法:采用蛋白质印迹法(Western Blot)的实验方法检测多药耐药相关蛋白(multidrug resistanceassociated protein) P-gp、LRP、MRP1及COX-2的表达。结果:肠复康方能够使相关蛋白P-gp、LRP、MRP1及COX-2的表达发生明显的下降,差异有统计学意义(P <0. 01)。结论:肠复康在相当程度上会促使P-gp、LRP、MRP1、COX-2的表达呈下调的趋势,考虑可能与大肠癌多药耐药的逆转机理有一定的联系。

Objective This study using the research methods of serum pharmacology and molecular biology to explore that the reversal effect of traditional Chinese medicine Changfukang for the multidrug resistance of clolrectal cancer,and its reversal mechanism,to provide research basis for its application in the clinical treatment of colorectal cancer. Methods Western blot method was used to detect the expression of colorectal cancer multidrug resistance-associated protein P-gp、MRP1、LRP and COX-2 to Changfukang.Results Changfukang can significant decrease the expression of resistance-associated protein p-gp、MRP1、LRP、and COX-2,the different was statistically significant( P < 0. 01). Conclusion The study showed that Chang Fu Kang can reduce the expression of colorectal cancer multidrug resistance protein P-gp、LRP、MRP1 and COX-2,It mainly reversed the mechanism of multidrug resistance of colorectal cancer.