流式细胞仪流动室多参数仿真优化研究

Multi Parameters Simulation and Optimization in Flow Chamber of Flow Cytometry

针对现有流式细胞仪样品液聚焦效果不良的现状,提出鞘液入口错位结构以改善聚焦效果,并提出利用截面密度的变化来确定样品液的聚焦直径。分别对鞘液入口速度和错开程度两个影响聚焦效果的因素进行分析。然后结合改进的坐标轮换法对仪器流动室2个重要参数进行联合仿真优化。最后利用流量守恒计算出鞘液的流速与仿真模型得到的流速对比,验证了仿真模型的适用及可靠性。优化结果表明:鞘液入口流速在一定范围内增大时,样品液聚焦直径逐渐减小;鞘液入口错开程度在(0～2.043)mm之间增加时,聚焦直径随之逐渐减小,超过2.043mm后,聚焦直径明显增大。经优化改进后的流动室对样品液的聚焦效果有明显的提升,其聚焦直径减小了64.14%,明显提高了细胞检测的准确率。

According to the adverse focusing effect of sample liquid of the traditional flow cytometry currently, establishing the model of sheath liquid entrance dislocation to improve the focusing effect, and the change of cross section density is used to determine the focus diameter of the sample solution. First of all, the factors of sheath entrance velocity and dislocation degree are analyzed, which affect the focusing effect. Then combine with the improved coordinate rotation method to simulate and optimize the two important parameters of flow chamber. At last, comparing with the data obtained from calculation by flow conservation and simulation model, the applicability and reliability of the simulation model is verifined. The optimization results show that with the increase of the inlet velocity in a certain range, the focusing diameter of the sample fluid decreases gradually. When the inlet dislocation degree of the sheath fluid increases from 0 to 2.043mm, the focusing diameter decreases gradually, and when it is over 2.043mm, focusing diameter increases significantly. After the optimization, the modified flow chamber structure has an obvious improvement on the focusing effect of the sample liquid, and the focus diameter is decreased by 64.14%,the accuracy of cell detection is improved Significantly.