疏血通注射液体外抗栓及抗凝作用研究

In Vitro Evaluation of Antithrombotic and Anticoagulant Effects of Shuxuetong Injection

目的探讨疏血通注射液(以下简称疏血通)体外抗凝、抗栓作用。方法 2015年5—7月,收集来自江苏省血液研究所的6例健康献血者的外周血,采用同一健康献血者的血液或血浆,以疏血通1份+血液或血浆9份作为加药组,0.9%氯化钠溶液1份+血液或血浆9份作为对照组。检测两组血常规指标、凝血指标、纤溶指标、血小板功能、体外血栓形成情况。结果对照组白细胞计数、血小板计数高于加药组(P<0.05)。两组红细胞计数、红细胞比容比较,差异无统计学意义(P>0.05)。加药组活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间(TT)长于对照组(P<0.001);对照组与加药组血块收缩率比较,差异无统计学意义(P>0.05)。纠正试验的APTT、PT、TT长于对照组(P<0.05);纠正试验的APTT、PT、TT短于加药组(P<0.05)。加药组D-二聚体、组织纤溶酶原激活物(t-PA)高于对照组,纤酶蛋白原(FIB)、优球蛋白溶解时间(ELT)、纤溶酶原激活物抑制剂1(PAI-1)低于对照组(P<0.05)。加药组ADP、瑞斯托霉素、胶原、凝血酶诱导的血小板聚集率均低于对照组(P<0.05);两组血小板黏附率比较,差异无统计学意义(P>0.05);加药组P-选择素高于对照组(P<0.05)。加药组R值、K值长于对照组,α角、MA值小于对照组(P<0.05)。结论疏血通在体外实验中显示出一定的抗凝、促纤溶、抑制血小板聚集和抑制血栓形成的作用。

ObjectiveTo investigate the in vitro anticoagulant and thrombolytic effects of Shuxuetong Injection.MethodsThe human peripheral blood from six healthy donors were collected in Institute of Hematology of Jiangsu Province from May to July 2015.The blood or plasma of the same healthy donors were used and divided into dosing group with 1 ∶ 9 volume ratio of Shuxuetong and blood or plasma（9 samples）,and control group with 1 ∶ 9 volume ratio of 0.9% sodium chloride solution and blood or plasma（9 samples）.Blood parameters,coagulation index,fibrinolysis index,platelet function,and thrombus in vitro of dosing group and control group were evaluated.ResultsLeukocyte count and platelet count of control group were higher than those of dosing group（P〈0.05）.Erythrocyte count and hematocrit of two groups had no significant difference （P〉0.05）.Activated partial thromboplastin time（APTT）,prothrombin time（PT） and thrombin time（TT） of dosing group were longer than those of control group（P〈0.001）.There was no significant difference in incidenca rate of clot retraction between two groups（P〉0.05）.APTT,PT and TT in control group were longer than those before correction（P〈0.05）,and these in dosing group were shorter than those before correction（P〈0.05）.The amount of D-dimer and t-PA in dosing group were higher than those in control group,and the amount of fibrinogen（FIB）,euglobulin lysis time（ELT） and PAI-1 were lower than those in control group（P〈0.05）.Platelet aggregation rate induced by ADP,ristomycin,collagen and thrombin in dosing group were higher than those in control group（P〈0.05）.There was no significant difference in platelet adhesion rate between two groups（P〉0.05）.The expression of P-selectin in dosing group was higher than that of control group（P〈0.05）.The R time and K time of dosing group were longer than those of control group,and α-angle and maximum amplitude were lower than those of control group（P〈0.05）.ConclusionShuxuetong Injection can have the effect of anti-coagulation,pro-fibrinolysis,anti-platelet aggregation and thrombosis inhibition in vitro.