电针远端取穴对神经根型颈椎病模型大鼠镇痛相关因子影响

Effects of Electroacupunctural Distal Acupoints on Analgesic Factors of Cervical Spondylosis Radiculopathy Model’s Rats

目的：观察电针远端取穴对神经根型颈椎病（CSR）模型大鼠IL-1β、IL-6的影响,探讨电针远端取穴对CSR模型大鼠的镇痛机制。方法：SPF级Wistar大鼠随机分为3组：空白组、模型组、电针组,每组各20只。除空白组除外,余两组采用手术方法将尼龙渔线放至大鼠C6～7、T1神经根腋下造成CSR模型。电针组大鼠取双侧＂太冲＂＂合谷＂电针,每天1次,每次20 min。干预7 d后检测各组大鼠机械痛阈。采用ELISA法、Real-Time PCR法分别检测压线处脊髓和神经根组织IL-1β、IL-6含量及IL-1βmRNA、IL-6 mRNA表达。结果：模型组痛阈较空白组显著降低（P〈0.05）;干预后电针组痛阈较模型组显著升高（P〈0.05）。模型组IL-1β、IL-6含量及其mRNA含量较空白组显著升高（P〈0.05）;电针组较模型组IL-1β、IL-6含量及其mRNA含量显著降低（P〈0.05）。结论：电针对CSR模型大鼠具有镇痛作用,通过降低脊髓及神经根组织IL-1β、IL-6含量和mRNA表达可能是其镇痛机制之一。

Objective：To observe the effects of electroacupunctural distal acupoints on analgesic factors of cervical spondylosis radiculopathy model＇s rats,and to discuss its analgesic mechanism. Methods：Randomly divided SPF Wistar rats into 3 groups,blank group,model group,and electroacupuncture group（n=20）. Excepted the blank group,put nylon fishing line into rats＇ C6-7,T1 nerve roots with operational method to simulate CSR model. Treated electroacupuncture group＇s rats with electroacupuncture at bilateral LR3 and LI4,20 minutes each time,once a day. Measured mechanical pain threshold of 3 groups of rats after 7-day-treatment. Detected contents of IL-1β,IL-6 in spinal cord and nerve tissue by ELISA,and determined espression of IL-1βmRNA,IL-6 mRNA by Real-Time PCR. Results：Mechanical pain threshold significantly decreased more in the model group than in the blank group（P〈0.05）. After treatment,electroacupunture group got a more significantly increase in mechanical pain threshold than model group（P〈0.05）. Compared with the blank group,contents of IL-1β and IL-6 as well as espressions of IL-1βmRNA and IL-6 mRNA in spinal cord and nerve tissue increased significantly in the model group（P〈0.05）. Compared with the model group,contents of IL-1β and IL-6 as well as espressions of IL-1βmRNA and IL-6 mRNA decreased significantly in the electroacupuncture group（P〈0.05）. Conclusion：Electroacupunctural distal acupoints impacts an analgesic effect on CSR-model＇s rats. Electroacupuncture treatment can reduce contents of IL-1β and IL-6 as well as espressions of IL-1βmRNA and IL-6 mRNA in rats＇ spinal cord and nerve tissue,it may be one of its analgesic mechanism.