苯甲酸、凝结芽孢杆菌和牛至油复合添加剂对大肠杆菌攻毒仔猪生长性能、抗氧化能力和空肠消化吸收功能的影响

Effects of Compound Additive with Benzoic acid,Bacillus coagulans and Oregano Oil on Growth Performance,Antioxidant Capacity and Jejunal Digestion-Absorption Function of Piglets Challenged with Enterotoxigenic Escherichia coli

本试验旨在研究苯甲酸、凝结芽孢杆菌和牛至油复合添加剂对大肠杆菌攻毒仔猪生长性能、抗氧化能力、空肠黏膜二糖酶活性和养分转运载体m RNA表达的影响。选取20头平均体重(7.64±0.46)kg健康的(24±1)日龄"杜×长×大"断奶仔猪,随机分为4组,每组5个重复,每个重复1头猪。对照组(CON组)和大肠杆菌组(ETEC组)饲喂基础饲粮,抗生素组(AT组)和复合添加剂组(ABO组)分别饲喂在基础饲粮中添加抗生素(20 g/t硫酸黏菌素+40 g/t杆菌肽锌)和复合添加剂(3 000 g/t苯甲酸+400 g/t凝结芽孢杆菌+400 g/t牛至油)的试验饲粮。试验第22天,ETEC、AT和ABO组仔猪灌服含3×1011CFU大肠杆菌的培养液,CON组仔猪灌服相同剂量的无菌培养液。试验期共26 d。结果表明:与CON组相比,ETEC组仔猪腹泻率和腹泻指数显著提高(P<0.05),血清和空肠黏膜丙二醛(MDA)含量显著提高(P<0.05),血清总抗氧化能力(T-AOC)和总超氧化物歧化酶(T-SOD)活性以及空肠黏膜钠-葡萄糖共转运载体1(SGLT1)m RNA表达水平显著降低(P<0.05),空肠黏膜T-AOC和T-SOD活性有降低的趋势(P<0.10)。与ETEC组相比,ABO组仔猪平均日增重(ADG)显著提高(P<0.05),仔猪料重比(F/G)显著降低(P<0.05),仔猪腹泻率和腹泻指数显著降低(P<0.05),血清和空肠黏膜MDA含量显著降低(P<0.05),血清和空肠黏膜T-AOC和T-SOD活性显著提高(P<0.05),空肠黏膜SG LT1和寡肽转运蛋白1(Pep T1)m RNA表达水平显著提高(P<0.05)。此外,与AT组相比,ABO组仔猪腹泻指数显著降低(P<0.05),血清T-AOC显著提高(P<0.05)。综上所述,饲粮添加苯甲酸、凝结芽孢杆菌和牛至油复合添加剂可显著缓解大肠杆菌攻毒诱导的仔猪腹泻,提高仔猪抗氧化能力,改善仔猪的生长性能和肠道消化吸收功能。

This experiment was conducted to investigate the effects of compound additive with benzoic acid, Bacillus coagulans and oregano oil on growth performance, antioxidant capacity, jejunal mucosa disaccharidase activities and nutrient transporter mRNA expression of piglets challenged with enterotoxigenic Escherichia coli . Twenty （24±1）-day-old Duroc×Landrace×Yorkshire castration piglets with body weight of （7.64±0.46） kg were randomly assigned to four groups with five replicates per group and one pig per replicate. Piglets in control group （CON group） and enterotoxigenic Escherichia coli -challenged group （ETEC group） were fed basal diets, and others in antibiotic group （AT group） and compound additives group （ABO group） were fed basal diets supplemented with antibiotics （20 g/t colistin sulfate＋40 g/t bacitracin zinc） and compound additives （ 3 000 g/t benzoic acid＋400 g/t Bacillus coagulans ＋400 g/t oregano oil）, respectively. On day 22, all piglets were orally infused with 3×10^11 CFU Escherichia coli , except piglets in CON group, which were received the same dose of sterile essential medium. The experiment lasted for 26 days. The results showed that compared with CON group, the diarrhea rate and diarrhea index of piglets in ETEC group were significantly increased （ P 〈 0.05）, the content of malondialdehyde （MDA） in serum and jejunal mucosa was significantly increased（ P 〈0.05）, the total antioxidant capability （T-AOC） and total superoxide dismutase（T-SOD）activity in serum and jejunal mucosa sodium-glucose cotransporter 1 （ SGLT1 ） mRNA expression levels were significantly decreased （ P 〈0.05）, and tended to decrease the T-AOC and the T-SOD activity in jejunal mucosa （ P 〈0.10）. Compared with ETEC group, the average daily gain （ADG） of piglets in ABO group was significantly increased （ P 〈0.05）, the feed to gain ratio （F/G） of piglets was significantly decreased （ P 〈0.05）, the diarrhea rate and diarrhea index of piglets were significantly decreased （ P 〈0.05）, the content of MDA in serum and jejunal mucosa were significantly decreased （ P 〈0.05）, the T-AOC and T-SOD activity in serum and jejunal mucosa were significantly increased （ P 〈0.05）, the jejunal mucosa SGLT1 and oligopeptide transporter 1 （ PepT1 ） mRNA expression level were significantly increased （ P 〈0.05）. Furthermore, compared with AT group, the diarrhea index of piglets in ABO group was significantly decreased （ P 〈0.05）, the serum T-AOC was significantly increased （ P 〈0.05）. In conclusion, dietary supplementation with the compound additive with benzoic acid, Bacillus coagulans and oregano oil can relieve the diarrhea of piglets challenged with Escherichia coli , improve the antioxidant capacity of piglets, and improve growth performance and jejunal digestion-absorption function.