摘要
本试验在合成二氢杨梅素-镍配合物(DMY-Ni)的基础上探讨其作为饲料添加剂的饲用安全性。以二氢杨梅素(DMY)和乙酸镍为原料,采用加热回流法合成DMY-Ni,通过紫外光谱、红外光谱对其进行表征。采用噻唑蓝(MTT)法,研究不同浓度(10、20、40、80和160μg/m L)、不同作用时间(12、24、36和48 h)下,DM Y与DM Y-Ni对小鼠正常肝实质细胞AM L12增殖的影响。结果显示:DM Y与镍离子配合后可生成DM Y-Ni。10、20和40μg/m L DM Y或DM Y-Ni分别作用AM L12细胞48 h后,AM L12细胞的存活率与空白对照组差异不显著(P>0.05);80μg/m L及以上浓度的DMY-Ni作用AML12细胞48 h后,AML12细胞的存活率显著低于空白对照组(P<0.01或P<0.001),160μg/m L的DMY作用AML12细胞48 h后,AM L12细胞的存活率显著低于空白对照组(P<0.01)。随DM Y或DM Y-Ni浓度的增加和作用时间的延长,AML12细胞的存活率逐渐降低。DMY与DMY-Ni对AML12细胞的半数抑制浓度(IC50)分别为285.10、222.84μg/m L。由此得出,DMY与DMY-Ni对AML12细胞都具有相对低毒性,且DMY-Ni对AML12细胞的毒性较DMY稍有增加。
This experiment was conducted to synthesis the dihydromyricetin-nickel complex (DMY-Ni) and clarify the feeding safety of DMY-Ni as a feed additive. DMY-Ni was prepared by the heating reflux method with dihydromyricetin (DMY) and nickel acetate as the materials. The complex was characterized by ultraviolet (UV) spectra and infrared (IR) spectra. DMY and DMY-Ni were used to study the effects of different concentrations (10, 20, 40, 80 and 160 μg/mL) and different action time (12, 24, 36 and 48 h) on the proliferation of normal liver parenchymal cells AML12 for mice by MTT method. The results showed as follows: the complex which was synthesized by DMY and nickel ion was confirmed as DMY-Ni. The survival rate of AML12 cells which were treated by 10, 20 and 40 μg/mL DMY or DMY-Ni for 48 h was not significantly different compared with the blank control group ( P 〉0.05). After treatment for 48 h, the survival rate of AML12 cells of DMY-Ni group of 80 μg/mL and above was significantly lower than that of the blank control group ( P 〈0.01 or P 〈0.001), and the survival rate of AML12 cells of DMY group of 160 μg/mL was significantly lower than that of the blank control group ( P 〈0.01). And the survival rate of AML12 cells was gradually decreased with the increase of the concentration of DMY or DMY-Ni and the extension of the action time. Half the inhibitory concentrations (IC 50 ) of DMY and DMY-Ni on AML12 cells were 285.10 and 222.84 μg/mL , respectively. In conclusion, DMY and DMY-Ni have relatively low toxicity to AML12 cells, and the toxicity to AML12 cells of DMY-Ni is slightly increased compared with DMY.
作者
张慧
郭清泉
舒绪刚
谢宇
谭威
刘迪
张亚楠
ZHANG Hui;GUO Qingquan;SHU Xugang;XIE Yu;TAN Wei;LIU Di;ZHANG Ya'nan(School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China;Guangzhou Tianke Biological Technology Co.,Ltd.,Guangzhou 510627,China;College ofZhongkai Agricultural Engineering,Guangzhou 510225,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第9期3734-3739,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
广州市科技计划项目产学研协同创新重大专项(201604020043)