饲料中添加牛磺酸对红鳍东方鲀幼鱼生长性能、体组成和肝脏中牛磺酸合成关键酶活性的影响

Effects of Dietary Taurine on Growth Performance,Body Composition and Activities of Key Taurine Synthesis Enzymes in Liver of Juvenile Tiger Puffer(Takifugu rubripes)

本试验旨在研究饲料中添加牛磺酸对红鳍东方鲀幼鱼生长性能、体组成和牛磺酸合成关键酶活性的影响。试验共配制4种等氮等脂的试验饲料,分别是含60%鱼粉的对照1饲料、含45%鱼粉的对照2饲料、含30%鱼粉且分别添加0.5%和1.0%牛磺酸的2种含牛磺酸饲料。选取健康、初始体重为(17.33±0.55) g的红鳍东方鲀幼鱼,随机分配到12个养殖桶中,每个养殖桶中投放25尾。将12个养殖桶随机分为4组,每组3个养殖桶,每组随机投喂1种试验饲料,持续投喂56 d。结果显示:1)各组存活率(SR)无显著差异(P>0.05);特定生长率(SGR)以1.0%牛磺酸组最高,对照2组最低,但各组间无显著差异(P> 0.05);对照1组和0.5%牛磺酸组的饲料效率(FE)显著高于对照2组(P<0.05),与1.0%牛磺酸组无显著差异(P>0.05);各组蛋白质沉积率(PPV)、蛋白质效率(PER)、肝体比(HSI)、脏体比(VSI)和肥满度(CF)均无显著差异(P>0.05)。2)各组血清和肝脏中谷丙转氨酶(GPT)和谷草转氨酶(GOT)活性均无显著差异(P>0.05)。各组肝脏中半胱氨酸双加氧酶(CDO)活性均无显著差异(P>0.05),对照2组肝脏中半胱亚磺酸脱羧酶(CSD)活性显著高于对照1组、0.5%牛磺酸组和1.0%牛磺酸组(P <0.05)。3)各组鱼体水分、粗蛋白质、粗脂肪和粗灰分含量均无显著差异(P> 0.05)。综合来看,饲料中添加0.5%或1.0%的牛磺酸可以减少30%的鱼粉用量而不显著影响红鳍东方鲀幼鱼的生长性能。

This experiment was investigated to study the effects of dietary taurine on grow th performance,body composition and activities of key taurine synthesis enzymes of juvenile tiger puffer（Takifugu rubripes）.Four isoenergetic and isonitrogenous experimental diets were formultated,they were control 1 diet containing 60%fish meal,control 2 diet containing 45% fish meal and two taurine diets containing 30% fish mean and adding0.5% and 1.0% taurine,respectively.Juvenile tiger puffers with the initial body weight of（17.33 ± 0.55） g were randomly assigned into 12 culture buckets and each buckets cultured 25 fish.The 12 culture buckets were randomly divided into 4 groups and each group had 3 culture buckets.Each diet was fed to one group for 56 d.The results showed as follow s：1） no significant differences were found in survival rate among groups（P〉0.05）.The specific grow th rate（SGR） of 1.0% taurine group was the highest,while the SGR of control 2 group was the lowest,but no significant difference was found among groups（P〉0.05）.The feed efficiency of control 1 group and 0.5% taurine group was significantly higher than that of control 2 group（P〈0.05）,but no significant difference was found when compared with 1.0% taurine group（P〉0.05）.No significant differences were found in protein productive value（PPV）,protein efficiency ratio（PER）,heaptosomatic index（HSI）,viscerosomatic index（VSI） and condition factor（CF） among groups（P〉0.05）.2） No significant differences were found in the activities of glutamic-pyruvic transaminase（GPT） and glutamic oxalacetic transaminase（GOT） in serum and liver among groups（P〉0.05）.No significant difference was found in the activity of liver cysteine dioxygenase（CDO） among groups（P〉0.05）.The activity of liver cysteinesulfinate decarboxylase（CSD） of control 2 group was significantly higher than that of control 1 group,0.5% taurine group and 1.0% taurine group（P〈0.05）.3） No significant differences were found in moisture,crude protein,crude lipid and ash contents of whole body among groups（P〉0.05）.Above results show that dietary 0.5% or 1.0%taurine can instead of 30% fish meal do not significantly affect the grow th performance of juvenile tiger puffer.