组织工程皮肤在复方黄芪口服液延缓皮肤衰老研究中的应用

Application of Tissue Engineering in Evaluating Anti-aging Effect of Compound Oral Liquid of Astragalus

为探究复方黄芪口服液对皮肤衰老的延缓功效,将SD大鼠随机分为含药血清组与不含药血清组,分别采用10mL/kg复方黄芪口服液和生理盐水灌胃SD大鼠7 d,每天1次,获取血清后将10%血清分别作用于200 nmol丝裂霉素(MMC)干预的人成纤维细胞衰老模型,验证了含药血清可有效促进细胞增殖.再利用组织工程皮肤Episkin表皮模型和三维真皮衰老模型,分别通过免疫荧光染色与Elisa试剂盒评价血清干预后表皮模型丝聚蛋白与三维真皮中人胶原蛋白I、III型的含量.实验结果表明,服用复方黄芪口服液有助于表皮层丝聚蛋白与真皮层人胶原蛋白I、III型蛋白的表达,从而提高皮肤屏障与保水能力,提升皮肤支撑力,减少皱纹生成,达到延缓皮肤衰老的功效.

To investigate the anti-aging effect of compound oral liquid of astragalus on skin, SD rats wererandomly divided into medicated serum group and non medicated serum group and given compound oral liquid ofastragalus and saline solution at the dose of 10 ml·kg^–1 once a day by intragastrical administration for 7 days,respectively. Firstly, 10 % serum was added to senescence of human fibroblasts model which had been treated with200 nM mitomycin （MMC） for 48 h. The experiment confirmed that medicated serum group has a significant rolein promoting the proliferation of fibroblasts. Secondly, the expression of filaggrin in Episkin and collagen types Iand III in three-dimensional dermal aging model was evaluated after being treated with serum byimmunofluorescence staining and Elisa kit, respectively. The results shows that compound astragalus oral liquid ishelpful to the expression of filaggrin in epidermis to improve the function of skin barrier and water retentioncapacity, and collagen types I and III to enhance mechanical properties of skin and reduce wrinkles. In conclusion,compound oral liquid of astragalus shows good effect of anti-aging on skin.