改良痈疽仙方油剂对中国白兔深Ⅱ度烫伤感染创面TNF-a、IL-8表达影响的实验研究

Experimental study on the effect of modified Zhijuxian formula oil on the expression of TNF-αand IL-8 in deep second degree scald infected wounds of Chinese white rabbits

目的探讨改良痈疽仙方油剂促进感染创面愈合的相关机制。方法选择30只中国白兔,制作深Ⅱ度烫伤并感染的创面,随机分为空白组、爱康肤银阳性对照组和改良痈疽仙方油剂组。创面分别给予相应处理,干预14天后,取部分创面组织,免疫组化观察TNF-a、IL-8值,比较3组差异。结果改良痈疽仙方油剂组白兔创面组织中TNF-a、IL-8值最低,与空白组比较具有显著差异性(P<0.01),与爱康肤银组比较无明显差异性(P>0.05)。结论改良痈疽仙方油剂可以有效下调白兔感染创面TNF-a、IL-8的表达,控制炎症的发展,其促进感染创面愈合的作用可能是通过此机制实现的。

Objective To investigate the mechanism of modified Zhijuxian formula oil in improving the healing of infected wounds.Methods Thirty Chinese white rabbits were prepared as model of deep II burn wound with infection and randomly divided into blank group,Aikang Fuyin positive control group and modified Zhijuxian decoction group. After 14 days of intervention,part of the wound tissues were taken and the values of TNF-α and IL-8 were observed by immune-histochemistry.Results The levels of TNF-α and IL-8 in the wound tissues of the rabbits in the modified Zhijuxian Formula oil group were the lowest,which had significant difference compared with the blank group（ P〈0. 01）,but there was no significant difference compared with Aikang Fuyin positive control group（ P〈0. 05）. Conclusions Modified Zhijuxian Formula oil could effectively down-regulate the expression of TNF-α and IL-8 in infected wounds of rabbits,and the mechanism may related to its effect in controlling the development of inflammation.