J亚群禽白血病病毒GDHX01株分离与基因序列分析

Isolation, Identification and Evolution Analysis of J Subgroup Avian Leukosis Virus GDHX01 Strain

为了解胡须鸡中是否存在禽白血病病毒(ALV)感染,从广东某胡须鸡养殖场中无菌采集胡须鸡血样,采用DF-1细胞培养、ELISA抗原检测、PCR扩增等方法,成功分离鉴定出一株J亚群禽白血病病毒(ALV-J),命名为GDHX01株。测序结果显示GDHX01株病毒基因组DNA序列全长为7616 bp,gp85基因全长919 bp。通过与其他禽白血病病毒参考毒株的基因组DNA序列进行比对分析,发现GDHX01与国内外J亚群禽白血病参考毒株同源性85.3%～95.7%之间。进一步比对发现,GDHX01毒株的gp85基因序列与国内外ALV-J参考株CAUGX01的同源性最高为95.8%。结果表明,在胡须鸡群中存在J亚群禽白血病病毒的感染。

In order to probe into whether there is avian leukosis virus （ALV）infection in Huxu chicken, the plasma samples were aseptically collected from a Huxu chicken farm in Guangdong and a ALV-J strain was successfully isolated and identified by DF-1 cell culture, ELISA antigen detection and PCR amplification, which is named GDHX01. The sequencing results showed that the genomic DNA sequence of GDHX01 strain was 7 616 bp in length. The gp85 gene of the GDHX01 isolate was 919 bp. By aligning with the genomic DNA sequences of other ALV reference strains. The GDHX01 had 85.3% to 95.7% nucleotide identity to ALV reference isolates. Further comparison showed that the gp85 sequence of GDHX01 isolate had the highest homology with the CAUGX01 strain of 95.8%. The results demonstrated that there was an infection of ALV-J in Huxu chicken.