UPLC法测定不同产地淫羊藿中11种成分的含量

Simultaneous Determination of 11 Compounds in Epimedium of Different Places of Origin by UPLC

目的：建立UPLC法用于同时测定不同产地淫羊藿中绿原酸、隐绿原酸、木兰花碱、金丝桃苷、朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、箭霍苷A、箭霍苷B、宝霍苷I 11种成分的含量。方法：采用CORTECS○RUPLC○RC18色谱柱（2. 1 mm×100 mm,1. 6μm）,流动相为0. 1%甲酸水（A）-乙腈（B）;梯度洗脱,洗脱程序为：0～4 min,5%～20%B; 4～5 min,20%～25%B; 5～10 min,25%～25%B; 10～11 min,25%～40%B; 11～17 min,40%～48%B; 17～18 min,48%～5%B,流速0. 3 m L/min,检测波长270 nm,柱温40℃,对不同产地淫羊藿中11种成分进行含量测定。结果：上述11种成分线性关系良好,精密度、重复性,稳定性的RSD均低于5. 50%,加样回收率为92. 71%～107. 77%。含量测定结果表明,不同产地的淫羊藿中,各成分含量差别较大,但均以朝霍定A、朝霍定B、朝霍定C、淫羊藿苷含量较高（四川产地除外）。结论：本方法具有简单、快速、重复性好等特点,可用于淫羊藿的质量控制研究。

Objective： To establish a UPLC method for simultaneous determination of baoxvoside I,chaoxing A,chaoxing B,chaoxing C,arrowthoside A,aristolone B,hyperoside,chlorogenic acid,magnolia alkali,cryptogenic acid and icariin in Epimedium of different places of origin. Methods： The compounds were separated on a CORTECS○RUPLC○RC18 column（2. 1 mm × 100 mm,1. 6 μm） at the temperature of 40 ℃. The mobile phase consisted of 0. 1 % formic acid in water and acetonitrile with a flow rate of 0. 3 m L/min. The gradient of B was 0-4 min,5%-20% B; 4-5 min,20%-25% B; 5-10 min,25%-25% B; 10-11 min,25%-40% B; 11-17 min,40%-48% B; 17-18 min,48%-5% B. Detection wavelength was set at 270 nm. Results： All compounds showed good linearity,the RSD of the precision,repeatability and stability tests were less than 5. 50%,and the average recoveries were ranged in 92. 71% ～ 107. 77%. The results showed that the content of compounds in Epimedium of different places of origin was different. The contents of chaoxing A,chaoxing B,chaoxing C and icariin were higher（except Sichuan origin）. Conclusion： The method was simple and rapid with high precision and repeatability,which can be used for the determination of 11 compounds in Epimedium.