DTX复合麻醉剂对獐血液学参数和氧化应激的影响

The effect of DTX compound anesthetic on hematological parameters and oxidative stress in Hydropotes

为探索DTX复合麻醉剂对獐血液学参数和体内氧化与抗氧化参数、血糖及离子浓度的影响,试验选取9头健康成年獐,按体重肌肉注射DTX复合麻醉剂0. 15 m L/kg,分别于麻醉前（注药前）、麻醉中（注药后30分钟）、苏醒后（注药后24小时）采集静脉血5 m L,取2 m L用EDTA-Li2抗凝,测定血液学指标,其余血样静置后分离血清测定氧化与抗氧化参数、血糖及离子浓度;监测麻醉各期时长;麻醉后每10 min监测一次围麻醉期呼吸频率（RR）、心率（HR）、收缩压（SAP）、舒张压（DBP）、直肠体温（RT）等呼吸和循环参数。结果表明：注射DTX复合麻醉剂后,獐血糖浓度升高,与麻醉前差异显著（P〈0. 05）,血清抗氧化酶谷胱甘肽过氧化酶（GSH-Px）和超氧化物歧化酶（SOD）活性显著升高（P〈0. 05）,但苏醒后均降至麻醉前水平,差异不显著（P〉0. 05）,丙二醛（MDA）含量升高,差异不显著（P〉0. 05）。全血红细胞数、白细胞数、血细胞比容、血红蛋白浓度等血液学参数在麻醉中有轻微降低,但无统计学差异（P〉0. 05）,血清AST、ALT活性和血中钙、钠、钾、氯、磷离子浓度无显著性变化（P〉0. 05）。麻醉诱导期持续（2. 9±0. 8） min,麻醉期持续（74±8） min,苏醒期持续（50±6） min。说明DTX复合麻醉剂对獐呼吸和循环功能影响轻微,未激发氧化应激反应,但能使獐血糖浓度升高,进而引起SOD和GSH-Px活性升高,因此对于高血糖动物使用DTX麻醉时需谨慎。

In order to investigate DTX compound anesthetic on hematological parameters and internal oxidation and antioxidant system in Hydropotes ,9 healthy adult Hydropotes were chosen in this experiment and were intramuscularly injected with 0.15 mL/kg of DTX compound anesthetic according to body weight. Each 5 mL of venous blood was collected before administration, 30 rain and 24 h post administration, in which 2 mL of blood samles supplemented with EDTA-Li2 for measuring hematological parameters and the remaining blood samples were left for determi- ning oxidation and antioxidant system parameters. Meanwhile, duration of each period of anesthesia was tested and, respiratory and circulatory parameters under perianesthesia including respiratory rate （ RR ）, heart rate （ HR ）, systemic blood pressure （ SAP ）, diastolic blood pressure （DBP） and rectal temperature（RT） were monitored every 10 min after anesthesia. The results showed that after DTX compound anesthetic was given, a significant increase in blood sugar concentration of Hydropotes appeared, which had significant difference with that before anesthesia （ P〈 0.05 ）, and the activities of GSH-Px and SOD had significantly rose（P〈0.05） ,however at 24 h post administration, those indexes decreased to the level before anesthesia and had no significant difference （P〉0.05）, and the content of malondialdehyde （MDA）had no significant difference （P〉O.05）. Hematological parameters including red blood cell count （RBC）, white blood cell count （WBC） ,haematocrit and hemoglobin had a slightly decrease after anesthesia but no significant difference （P〉 0.05）, while the activities of serum AST and ALT in sera, and blood ion concentrations of Ca2^＋ ,Na^＋ ,K^＋ ,Cl^- ,P had no significant changes（P〉0.05）. Anesthesia induction period lasted （2.9±0.8） min,anesthesia period lasted （74± 8）min, and recovery period lasted （50±6）min. The results suggested that DTX compound anesthetic had a slight effect on respiratory and circulatory function, and no oxidative stress was induced, while that made blood sugar concentration of Hydropotes rise and a significant increase in the activities of SOD and GSH-Px,therefore the caution should be exercised when using DTX in hyperglycemic animals.