MiR-29a通过调节HuR影响前列腺癌细胞的生物学行为

MiR-29a affects the biological behavior by regulating HuR in prostate cancer cells

目的 研究前列腺癌中miR-29a对HuR表达的调节,进而影响细胞增殖、迁移、侵袭、凋亡.方法 通过实时荧光定量（qRT-PCR）和免疫印迹法（Western blot）检测在正常前列腺上皮细胞RWPE-1和激素非依赖性前列腺癌细胞PC-3内miR-29a、人抗原R（HuR） mRNA和蛋白的表达水平;miR-29a mimics（模拟剂）、inhibitor（抑制剂）和NCmiRNA（对照剂）转染PC-3细胞48h后检测miR-29a、HuR mRNA和蛋白的表达水平.细胞增殖试剂盒（CCK-8）、迁移和侵袭实验（Transwell小室）、流式细胞术分别检测PC-3细胞被转染后细胞增殖、迁移侵袭、凋亡的改变.结果 与RWPE-1细胞相比,PC-3细胞中miR-29a的表达下调,HuR mRNA和蛋白的上升（P＜0.05）.与对照组相比,miR-29a mimics（模拟剂）、inhibitor（抑制剂）转染PC-3细胞后HuR mRNA无改变,但是能够改变HuR蛋白的表达（P＜0.05）.MiR-29a能够抑制细胞增殖、迁移、侵袭,促进细胞凋亡（P＜0.05）.结论 在前列腺癌PC-3细胞中,miR-29a通过调节HuR蛋白的表达,进而对细胞增殖、迁移、侵袭、凋亡产生影响.

Objective To investigate regulation of human antigens R （HuR） by miR-29a in prostate cancer and affect cell proliferation,migration,invasion and apoptosis.Methods The expression levels of miR-29a,HuR mRNA and protein between normal prostate epithelial RWPE-1 cells and hormone independent prostate cancer PC-3 cells were detected by qRT-PCR and Western blot.The expressions of miR-29a,HuR mRNA and protein were detected again after transfected with miR-29a mimics,inhibitor and negative control（NC） of PC-3 cells for 48 h.The cell proliferation kit （CCK-8）,migration and invasion assay （Transwell chamber） and Flow Cytometry were used to detect the change of PC-3 cells on proliferation,migration,invasion and apoptosis after transfection.Results Compared with RWPE-1 cells,miR-29a expression was down-regulated and HuR mRNA and protein were up-regulated in PC-3 cells（P ＜0.05）.Compared with NC,there was no change in HuR mRNA expression,but HuR protein was changed after transfected with miR-29a mimics or inhibitor （P ＜0.05）.MiR-29a had the function of inhibit cell proliferation,migration and invasion,and promote apoptosis（P ＜0.05）.Conclusions MiR-29a regulates the expression of HuR protein in PC-3 cells,which may affect cell proliferation,migration,invasion and apoptosis.