生殖道感染与不育男性精液NO、IL-17、IL-18浓度的相关性分析

Correlation analysis of serum nitric oxide concentration and IL-17 and IL-18 expression in sterile male

目的 探讨不育症患者精液中一氧化氮（NO）、IL-17和IL-18浓度与生殖感染的关系.方法 选择2014年1月至2016年2月在本院泌尿外科就诊的81例不育症患者（有无解脲脲原体感染）和正常男性作为研究对象,分为正常男性（A组）、无生殖道解脲支原体感染的不育症患者（B组）和生殖道解脲支原体感染的不育症患者（C组）,CASA法检测精子浓度、前向运动百分比、活动率,5g/L伊红染色检测精子存活率,巴氏染色计数精子正常形态率,检测精浆NO、IL-17和IL-18的浓度.结果 生殖道感染的不育症患者,其精液中一氧化氮浓度高于无生殖道感染的不育症患者和正常男性（P＜0.05）.解脲支原体感染的不育症患者的精子浓度、PH值、前向百分率、精子运动、精子活动率、精子存活率、精子正常形态率均显著低于无生殖道感染的不育症患者和正常男性（P＜0.05）.不育症患者精液中的NO浓度与IL-17和IL-18浓度呈正相关.结论 生殖道感染诱发精浆NO、IL-17和IL-18浓度升高,导致生殖能力损伤,从而引起男性不育.

Objective To research the relationship between genital infection and two sperm parameters,namely,concentrations of nitric oxide （NO） and [cytokines interleukin （IL-17 and IL-18）],in the semen of infertile males remains undetermined.Methods Semen samples from 81 infertile （with or without ureaplasma urealyticum infection） and normal males were subjected to semen analysis from January 2014 to February 2016 in our hospital.The sperm concentration,forward movement percentage,motility were detected by CASA method,and the viability was detected by 5 g/L staining,while papanicolaou staining was used to detect the normal sperm morphology rate.The concentration of NO,il-17 and il-18 was detected.Results Nitric oxide concentration in the semen of infertile males with genital infection was higher than those of infertile males without genital infection and of normal males （P ＜ 0.05）.Sperm density,PH,percentage of forward,movement of sperm,sperm activation rate,sperm survival rate,and normal rate of the sperm morphology were significantly lower than those of infertile males without genital infection and of normal males （P ＜ 0.05）.NO concentrations were also positively correlated with IL-17 and IL-18 concentrations in the semen of infertile males.Conclusions Increased NO concentration and abnormal IL-17 and IL-18 expression are induced by genital infection and induced damage to male reproductive capacity,thereby causing male infertility.