中药材等复杂基质中真菌毒素检测的前处理技术研究新进展

Research progress of pretreatment technology for mycotoxin detection in Chinese materia medica and complex matrices

中药材、食品、农产品等基质在种植、采收、加工、运输和贮藏过程中,若处理不当,极易污染产毒真菌而导致真菌毒素残留,可直接影响基质的质量与安全性和有效性,严重威胁消费者的身体健康和生命安全。建立高灵敏的分析方法可快速检测中药材等基质中真菌毒素的污染种类和残留水平,为降低真菌毒素的暴露风险提供依据。由于中药材和食品、农产品等基质的复杂性,高效快速的样品前处理技术是建立高灵敏的真菌毒素快速检测技术的前提和保障。该文对目前真菌毒素检测常用的提取和净化方法进行综述,阐述了前处理技术对中药材等复杂基质中真菌毒素精确定量的重要性,并展望了未来真菌毒素检测的样品前处理技术的发展趋势。

In the processes of planting,harvest,transport and storage,improper treatment of Chinese materia medica（ CMM） and foodstuffs and agricultural products will result in fungal growth and mycotoxins contamination,which will not only directly affect the quality,safety and efficacy of these complex matrices,but also seriously threaten the consumers＇ health and lives. Therefore,the establishment of high-throughout analytical methods with high sensitivity for the determination of mycotoxins in CMM and foodstuffs and agricultural products at trace levels will provide reliable references for reducing the risk of mycotoxin exposure in humans. Due to the matrix complexity of CMM and foodstuffs and agricultural products,highly-effective pretreatment technologies are necessary for the establishment of such analytical techniques. In this review,the current extraction and purification methods commonly used for the detection of mycotoxins were summarized,the importance of pretreatment techniques for the precise quantification of mycotoxins in complex matrices such as Chinese herbal medicines was highlighted,as well as the development tendency about the pretreatment techniques for mycotoxins in complex matrices in the future was proposed.