维生素D防治慢性胃炎小鼠幽门螺杆菌感染的研究

Prevention and treatment and its mechanism on Helicobacter pylori infection in mice with chronic gastritis by vitamin D

目的探讨维生素D在幽门螺杆菌感染小鼠体内防治幽门螺杆菌作用及其作用机制。方法野生型C57BL/6J小鼠随机分为4个实验组和2个对照组,每组各6只。实验组ig幽门螺杆菌浓度至少5×10~8 CFU/mL磷酸盐缓冲液建立慢性胃炎模型,分别ig维生素D 0.003、0.012、0.048μg/d(1、4、16倍剂量),处理12周。处死小鼠,留取胃组织进行组织病理学、免疫组织化学和RT-PCR检测p53、NF-κB p65基因表达。结果 0.012、0.048μg/d维生素D能减少小鼠幽门螺杆菌定植、炎症细胞浸润和炎症反应,能增加p53基因表达,降低了NF-κB p65基因表达(P<0.05),并呈剂量相关性,但在0.012、0.048μg/d组间差异无统计学意义。结论大剂量维生素D可以促进p53表达,降低NF-κB p65表达,具有防治幽门螺杆菌感染,减轻幽门螺杆菌感染相关炎症反应的作用。

Objective To discuss prevention and treatment of vitamin D on Helicobacter pylori in mice in vivo, and explore its mechanism. Methods Wild type C57 BL/6 J mice were randomly divided into 4 experiment groups and 2 control groups, and each group had 6 mice. Mice were ig administration with H. pylori above 5×10~8 CFU/mL phosphate buffer solution to establish chronic gastritis model. Mice in experiment groups were ig administration with 0.003, 0.012, and 0.048 μg/d（1, 4, and 16 times dose） for 12 weeks. The mice were sacrificed, and gastric tissues were collected. Histopathological and immunohistochemical results were observed, and expressions of p53 and NF-κB p65 were determined by RT-PCR method. Results Vitamin D（0.012 and 0.048 μg/d） could reduce the colonization of H. pylori, inflammatory cell infiltration, and inflammatory reaction, increase the expression of p53 gene, and decrease the expression of NF-κB p65 gene in a dose dependent manner in mice（P〈0.05）, but there was no significant difference between two groups. Conclusion The high dose of vitamin D treated has anti Helicobacter pylori in mice. High dose of vitamin D can promote the expression of p53 and reduce the expression of NF-κB p65. It can prevent and treat H. pylori infection and alleviate the inflammation related to H. pylori infection.