细胞粘附肽Arg-Gly-Asp的合成与生物活性检测

The synthesis of cell adhesion peptide Arg-Gly-Asp and biology activity examination

采用固相多肽合成法合成Ag-G ly-Asp(RGD)三肽,以天门冬氨酸计RGD三肽收率为75%,采用MTT法进行合成肽RGD抑制人纤维肉瘤细胞HT1080转移机理方面的研究,证实了人工合成的RGD肽能抑制人纤维肉瘤细胞HT1080与纤维连接蛋白(FN)的粘附。

Solid phase method is used to synthesize RGD in this paper,the yield is 75%calculated from the first amino acid anchored resin.The mechanism of synthesized peptide RGD on the metastasis of human fibrosarcoma cell HT 1080 is studied by MTT method,It shows that the synthesized polypeptide RGD can inhibit the adhesion between HT1080 and FN.