miRNA-124在上皮性卵巢癌组织中的表达及临床意义

Expression and clinical significance of miRNA-124 in epithelial ovarian cancer

目的探讨miRNA-124在上皮性卵巢癌组织中的表达及临床意义。方法选择2013年2月至2014年11月在浙江省台州医院就诊的上皮性卵巢癌组织85例、良性上皮卵巢肿瘤组织35例和正常卵巢组织45例作为研究对象。采用实时荧光定量逆转录-聚合酶链式反应(RT-PCR)检测组织中miRNA-124的表达水平,分析miRNA-124的相对表达量与临床病理特征及预后的关系。结果卵巢癌组织中miRNA-124相对表达量为2.35±1.11,良性卵巢肿瘤组织中的表达量为5.13±2.01,正常组织中为6.29±2.12。卵巢癌组织中miRNA-124相对表达量明显低于良性卵巢肿瘤组织和正常组织(t值分别为7.123、11.650,均P<0.001);良性卵巢肿瘤组织中miRNA-124相对表达量低于正常组织(t=2.499,P=0.007)。临床分期和组织分级越高,miRNA的相对表达量越低(t值分别为13.581、23.232,均P<0.001);有淋巴结转移者,miRNA的相对表达量越低(t=11.671,P<0.001);组织学类型与miRNA的相对表达量无关(t=1.003,P>0.05)。高表达组的总生存率明显高于低表达组,差异有统计学意义(χ~2=7.824,P=0.008)。低表达量组中位生存期为(7.93±1.54)个月,明显低于高表达量组的(14.51±6.72)个月,差异有统计学意义(t=6.053,P<0.001)。结论上皮性卵巢癌组织中miRNA-124的相对表达量降低,提示其可能在卵巢癌的发病机制中起到抑癌基因的作用,另外,miRNA-124的表达量与卵巢癌的进展和转归有关。

Objective To investigate the expression and clinical significance of miRNA-124 in epithelial ovarian cancer.Methods There were 85 cases of epithelial ovarian cancer visiting Taizhou Hospital,35 cases of benign epithelial ovarian tumor and 45 cases of normal ovarian tissue included in this study from February 2013 to November 2014.Real-time fluorescence quantitative reverse transcription polymerase chain reaction（RT-PCR）was used to detect the expression of miRNA-124 in the tissues.The relationship between the relative expression of miRNA-124 and the clinicopathological features and prognosis was analyzed.Results The relative expression of miRNA-124 in ovarian cancer tissues was 2.35±1.11,that in benign ovarian tumors was 5.13±2.01,and that in normal tissues was 6.29±2.12,respectively.The relative expression of miRNA-124 in ovarian cancer tissue was significantly lower than in benign ovarian tumor tissues and normal tissue（t value was 7.123 and 11.650,respectively,both P0.001）.The relative expression of miRNA-124 in benign ovarian tumor tissues was lower than in normal tissues（t=2.499,P=0.007）.The higher the clinical stage and histological grade,the lower the relative expression of miRNA（t value was 13.581 and 23.232,respectively,both P0.001）.The relative expression of miRNA was lower in patients with lymph node metastasis（t=11.671,P0.001）,and the histological type was not related to the relative expression of miRNA-124（t=1.003,P〈0.05）.The total survival rate of the high expression group was significantly higher than the low expression group,and the difference was statistically significant（χ~2=7.824,P =0.008）.The median survival time of the low expression group was 7.93±1.54 months,which was significantly lower than the high expression group（14.51±6.72 months）,and the difference was statistically significant（t=6.053,P0.001）.Conclusion The relative expression of miRNA-124 is decreased in epithelial ovarian cancer,suggesting that it may play a role in suppressing gene in the pathogenesis of ovarian cancer.In addition,the expression of miRNA-124 is related to the progression and prognosis of ovarian cancer.