樱桃谷鸭胚胎期毛囊发育miRNA及相关基因表达研究

MicroRNAs profiling and gene expression during embryonic periods of Cherry Valley duck

为揭示鸭羽毛发育的遗传机制,选取樱桃谷鸭胚胎期第11、15和20天和初生后第1天皮肤样品(每组3个重复),建立miRNA表达谱文库。根据胚胎期不同胚龄和初生后表达量变化特征和差异,采用qRT-PCR法验证差异表达的miRNA在组织中的表达量。从miRNA表达量上分析影响羽毛发育调控的关键基因,并采用Proc CORR分析差异表达miRNA与羽毛发育相关基因的关联性。结果表明:试验共获得337个miRNAs,其中差异表达的miRNAs216个,新miRNAs 93个。PI3K信号系统的雷帕霉素靶蛋白(mTOR)参与毛囊发育调控。miR-30a-5p和miR-203a表达量与Fzd3表达量呈极显著负相关,miR-novel-20表达量与Wnt8a和Birc6表达量呈显著或极显著负相关。这一研究提示,在胚胎发育早期,Wnt信号途径就开始参与毛囊生长的调控过程。

In order to elucidate the genetic mechanism of feather development in duck,skin samples at their embryonic day of 11,15,and 20,and the first day after hatch（1 D）were collected（3 replicates in each time point）for miRNA expression library in Cherry Valley ducks.Three miRNAs with differential expression patterns at their four selected stages were selected for QRT-PCR verification.Candidate genes regulating feather development were predicted according to base pairing of miRNA and target gene miRNA.The correlation between differentially expressed microRNAs and candidate genes was analyzed by Proc CORR.The results showed that 337 miRNAs were obtained with 216 differentially expressed miRNA and 93 new miRNAs.Rapamycin target protein（mTOR）in the PI3 Ksignaling pathway was involved in the regulation of feather follicle development.The expression of miR-30 a-5 pand miR-203 a was negatively correlated with the expression Fzd3.The expression of miR-novel-20 was negatively correlated with expression of Wnt8 aand Birc6.This study suggests that Wnt signaling pathway is involved in the regulation of feather follicle growth at the early stage of embryo development.